High Speed Separation of Therapeutic mAb
Columns: |
TSKgel SuperSW mAb HTP
4 µm, 4.6 mm ID × 15 cm |
Mobile phase: |
0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3 |
Flow rate: |
0.50 mL/min, 0.35 mL/min |
Detection: |
UV @ 280 nm |
Temperature: |
25 °C |
Injection vol.: |
10 µL
|
Sample: |
monoclonal antibody-2 (mouse-human chimeric IgG, Erbitux®),
5 µL |
|
|
Separation of Four Nucleobases Using TSKgel SuperSW mAb HTP Column in HILIC mode at pH 7.4
Column: |
TSKgel SuperSW mAb HTP
4 µm, 4.6 mm ID × 15 cm
|
Mobile phase: |
A: acetonitrile
B: 15 mmol/L ammonium bicarbonate, pH 7.4 |
Gradient: |
isocratic |
Flow rate: |
0.4 mL/min |
Detection: |
UV @ 280 nm |
Injection vol.: |
1 µL
|
Temperature: |
ambient |
Samples: |
uracil (1.5 g/L), adenine (1.5 g/L), cytosine (1.5 g/L), cytodine (1.5 g/L), from Sigma Aldrich |
|
For additional applications, please see our applications database.