HPLC Columns

TSKgel G2000SW

As the first generation of silica-based SEC columns, TSKgel G2000SW columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.

TSKgel G2000SW columns are available in 10 and 13 μm for analytical and semi-preparative purifications.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0005788TSKgel G2000SW10Stainless Steel7.5301,987
0005102TSKgel G2000SW10Stainless Steel7.5603,251
0006727TSKgel G2000SW13Stainless Steel21.5305,521
0005146TSKgel G2000SW13Stainless Steel21.5608,023

Product Attributes

Base material: Silica
Particle size (mean): 10 µm, 13 µm
Pore size (mean): 12.5 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 5,000 - 1.0 × 10Da
(globular proteins)


Calibration Curves for Globular Proteins, Polyethylene Oxides and Dextrans
for TSKgel SW Columns


Columns: TSKgel SW columns, 7.5 mm ID × 60 cm × 2
Mobile phase: dextrans and polyethylene oxides: distilled water;
proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm and RI
Temperature: A. 25°C  B. 25°C  C. 40°C
Samples: proteins (black circle),
polyethylene oxides (white circle),
dextrans (half circle)


Separation of Nucleic Acid




Column: TSKgel G2000SW, 10 µm,
7.5 mm ID × 60 cm
Mobile phase: acetic acid/triethylamine/H2O = 3/3/94
Flow rate: 0.74 mL/min
Detection: UV @ 260 nm
Sample: 1. uridine
2. uracil
3. thymine
4. adenosine
5. adenine

Comparison of the Pore Sizes of TSKgel SW Packings for the Separation of a Protein Mixture


Columns: TSKgel SW columns, two 7.5 mm ID × 60 cm columns in series
Mobile phase: 0.3 mol/L NaCl in 0.05 mol/L phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Samples: 1. thyroglobulin, 2. bovine serum albumin, 3. β-lactoglobulin, 4. myoglobin, 5. cytochrome C, 6. glycine tetramer


For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1005 TSKgel G2000SW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
SR024 Applications for High-Performance Gel Filtration Columns (TSKgel SW and TSKgel PW columns): Focus on BiopolymersSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0005371TSKgel G2000SW-G4000SW Guard Column for 7.5 mm ID columns10Stainless Steel7.57.5748
0005758TSKgel G2000SW-G4000SW Guard Column for 21.5 mm ID columns13Stainless Steel21.57.51676