HPLC Columns

TSKgel G2500PWxl

The TSKgel G2500PWxl column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 3,000 Da. Because of the presence of cationic groups on the base bead, this column is not suited for separating anionic polymers. The smaller particle size of the TSKgel G2500PWxl column provides 1.7× higher resolution than the TSKgel G2500PW column, making the TSKgel G2500PWxl column more suitable for analytical purposes.

The TSKgel G2500PWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008020TSKgel G2500PWxl7Stainless Steel7.8302,341

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 7 µm
Pore size (mean): 12.5 nm
Calibration range: <3,000 ​Da (polyethylene glycols and oxides)

Polyethylene Glycol and Oxide
Calibration Curves


Columns: A. G2500PWXL
B. G3000PWXL 
C. G4000PWXL
D. G5000PWXL 
E. G6000PWXL
all 7.8 mm ID x 30 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: Rl


Desalting of Nucelosides



Column: TSKgel G2500PWXL, 7 µm,
7.8 mm ID × 30 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Samples: 1. 0.5 mol/L NaCl
2. uridine
3. adenosine

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1022 TSKgel G2500PWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR037 Introduction of Aqueous SEC Columns: TSKgel PWxl series Separation Report
SR038 GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series ColumnsSeparation Report
TIS137 Measurement of Molecular Mass of Lignin by GPC with Aqueous SolutionTechnical Information Sheets
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008033TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns12Stainless Steel64798