HPLC Columns

TSKgel G3000PWxl

The TSKgel G3000PWxl column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 50,000 Da. The smaller particle size of the TSKgel G3000PWxl column provides 1.7× higher resolution than the TSKgel G3000PW column, making the TSKgel G3000PWxl column more suitable for analytical purposes.

The TSKgel G3000PWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008021TSKgel G3000PWxl7Stainless Steel7.8302,341

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 7 µm
Pore size (mean): 20 nm
Calibration range: <4.0 × 104 ​Da (polyethylene glycols and oxides)



PEG and PEO Calibration Curves


Columns: A. G2500PWXL
B. G3000PWXL
C. G4000PWXL
E. G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: Rl

Protein Calibration Curves


Columns: 1. TSKgel G3000PWXL
2. TSKgel G4000PWXL
3. TSKgel G5000PWXL
4. TSKgel G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.8
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: a. thyroglobulin (6.6 × 10Da)
b. γ-globulin (1.5 × 10Da)
c. albumin (6.7 × 10Da)
d. ovalbumin (4.3 × 10Da)
e. β-lactoglobulin (3.6 × 10Da)
f. myoglobin (1.69 × 10Da)
g. cytochrome C (1.24 × 10Da)



Analysis of Small Peptides



Column: TSKgel G3000PWXL, 6 µm,
7.8 mm ID × 30 cm
Mobile phase: 0.1% TFA/45% CH3CN
Flow rate: 1.0 mL/min
Samples: peptides
1. aprotinin
2. insulin β-chain
3. α-MSH
4. bradykinin potentiator C
5. glutathione


For additional applications, please see our applications database.

Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1023 TSKgel G3000PWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR037 Introduction of Aqueous SEC Columns: TSKgel PWxl series Separation Report
SR038 GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series ColumnsSeparation Report
TIS137 Measurement of Molecular Mass of Lignin by GPC with Aqueous SolutionTechnical Information Sheets
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008033TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns12Stainless Steel64798