HPLC Columns

TSKgel G4000PW

TSKgel G4000PW columns are ideally suited for the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides, with molecular weights of less than 300,000 Da.

Stable from pH 2 to 12, TSKgel G4000PW columns can be used in mobile phases of water or buffer (up to 20% methanol/80% aqueous) and can tolerate temperatures up to 80 °C.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0005763TSKgel G4000PW17Stainless Steel7.5302,098
0005107TSKgel G4000PW17Stainless Steel7.5603,411

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 17 µm
Pore size (mean): 50 nm
Calibration range: Up to 3.0 × 105​ Da (polyethylene glycols and oxides)

Polystyrene Calibration Curves


Columns: A. G2000PW B. G2500PW
C. G3000PW D. G4000PW
E. G5000PW F. G6000PW
all 7.5 mm ID × 60 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: Rl


Analysis of Polysaccharides


Columns: TSKgel G6000PW + G4000PW, two 7.5 mm ID × 60 cm columns in series
Mobile phase: H2O with 0.2 mol/L NaCl
Flow rate: 0.9 mL/min
Temperature: 40 °C
Sample: hyaluronic acid, polyethylene oxide

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1017 TSKgel G4000PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR024 Applications for High-Performance Gel Filtration Columns (TSKgel SW and TSKgel PW columns): Focus on BiopolymersSeparation Report
SR035 GFC Analysis of Water-Soluble Polymers with TSKgel PW ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0006762TSKgel G2500PW-GMPW Guard Column for 7.5 mm ID columns12Stainless Steel7.57.5761