TSKgel G4000PWxl

The TSKgel G4000PWxl column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 300,000 Da. The smaller particle size of the TSKgel G4000PWxl column provides 1.7× higher resolution than the TSKgel G4000PW column, making the TSKgel G4000PWxl column more suitable for analytical purposes.

The TSKgel G4000PWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008022	TSKgel G4000PWxl	10	Stainless Steel	7.8	30	2,412	Inquire

Product Attributes

Base material:	hydroxylated polymethacrylate
Particle size (mean):	10 µm
Pore size (mean):	50 nm
Calibration range:	2,000 - 3.0 × 10⁵ Da (polyethylene glycols and oxides)

PEG and PEO Calibration Curves

Columns:	A. G2500PWXL B. G3000PWXL C. G4000PWXL D.G5000PWXL E. G6000PWXL F. GMPWXL all 7.8 mm ID × 30 cm
Mobile phase:	distilled H₂O
Flow rate:	1.0 mL/min
Detection:	Rl

Protein Calibration Curves

Columns:	1. TSKgel G3000PWXL 2. TSKgel G4000PWXL 3. TSKgel G5000PWXL 4. TSKgel G6000PWXL 5. TSKgel GMPWXL all 7.8 mm ID × 30 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.8
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Samples:	a. thyroglobulin (6.6 × 10⁵Da) b. γ-globulin (1.5 × 10⁵Da) c. albumin (6.7 × 10⁴Da) d. ovalbumin (4.3 × 10⁴Da) e. β-lactoglobulin (3.6 × 10⁴Da) f. myoglobin (1.69 × 10⁴Da) g. cytochrome C (1.24 × 10⁴Da)

Separation of Gelatin

Column:	A. TSKgel G6000PWXL B. TSKgel G5000PWXL C. TSKgel G4000PWXL; all 7.8 mm ID × 30 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.0
Flow rate:	1.0 mL/min
Detection:	RI
Sample:	gelatin

For additional applications, please see our applications database.

Code #	Description	Literature Type
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
DS1024	TSKgel G4000PWxl Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01B	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series Columns	Product Catalog
SR037	Introduction of Aqueous SEC Columns: TSKgel PWxl series	Separation Report
SR038	GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008033	TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns	12	Stainless Steel	6	4	822