HPLC Columns

TSKgel G4000PWxl

The TSKgel G4000PWxl column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 300,000 Da. The smaller particle size of the TSKgel G4000PWxl column provides 1.7× higher resolution than the TSKgel G4000PW column, making the TSKgel G4000PWxl column more suitable for analytical purposes.

The TSKgel G4000PWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008022TSKgel G4000PWxl10Stainless Steel7.8302,341

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 10 µm
Pore size (mean): 50 nm
Calibration range: 2,000 - 3.0 × 10​5​ Da (polyethylene glycols and oxides)



PEG and PEO Calibration Curves


Columns: A. G2500PWXL
B. G3000PWXL
C. G4000PWXL
E. G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: Rl

Protein Calibration Curves


Columns: 1. TSKgel G3000PWXL
2. TSKgel G4000PWXL
3. TSKgel G5000PWXL
4. TSKgel G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.8
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: a. thyroglobulin (6.6 × 10Da)
b. γ-globulin (1.5 × 10Da)
c. albumin (6.7 × 10Da)
d. ovalbumin (4.3 × 10Da)
e. β-lactoglobulin (3.6 × 10Da)
f. myoglobin (1.69 × 10Da)
g. cytochrome C (1.24 × 10Da)



Separation of Gelatin



Column: A. TSKgel G6000PWXL
B. TSKgel G5000PWXL
C. TSKgel G4000PWXL;
all 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.0
Flow rate: 1.0 mL/min
Detection: RI
Sample: gelatin


For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1024 TSKgel G4000PWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR037 Introduction of Aqueous SEC Columns: TSKgel PWxl series Separation Report
SR038 GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008033TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns12Stainless Steel64798