HPLC Columns

TSKgel G4000SW

As the first generation of silica-based SEC columns, the TSKgel G4000SW columns provide excellent separation of proteins and other biomolecules.

TSKgel G4000SW columns are available in 13 μm particle size in Glass and Stainless Steel analytical columns and 17 μm, 21.5 mm ID x 30 cm and 60 cm columns for semi-preparative purifications.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
05790TSKgel G4000SW13Stainless Steel7.5301,638
05104TSKgel G4000SW13Stainless Steel7.5602,680
06729TSKgel G4000SW17Stainless Steel21.5304,553
05148TSKgel G4000SW17Stainless Steel21.5606,617

Product Attributes

Base material: Silica
Particle size (mean): 13 µm,17 µm
Pore size (mean): 45 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 2.0 × 104 - 7.0 × 10Da (globular proteins)



Calibration Curves for Globular Proteins, Polyethylene Oxides and Dextrans 
for TSKgel SW Columns


Columns: TSKgel SW columns, 7.5 mm ID × 60 cm × 2
Mobile phase: dextrans and polyethylene oxides: distilled water;
proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm and RI
Temperature: A. 25°CB. 25°CC. 40°C
Samples: proteins (black circle),
polyethylene oxides (white circle),
dextrans (half circle)



Separation of Total E. Coli RNA




Columns: TSKgel G4000SW, 13 µm,
7.5 mm ID × 30 cm × 2
TSKgel G4000SW, 17 µm,
7.5 mm ID × 30 cm × 2

Mobile phase: 0.13 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0, + 1 mmol/L EDTA
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Injection vol.: 5 µg
Sample: 0.1 mL of 1:10 diluted solution of total E. coli RNA:
1. 23s rRNA (1.1 × 106 Da)
2. 16s rRNA (5.6 × 105 Da)
3. 5s rRNA (3.9 × 104 Da)
4. 4s rRNA (2.5 × 104 Da)


Double Stranded DNA Fragments




Column: A: TSKgel G3000SW, 10 µm,
7.5 mm ID × 60 cm × 2
B: TSKgel G4000SW, 13 µm,
7.5 mm ID × 60 cm × 2
Mobile phase: 0.05 mol/L Tris-HCl, 0.2 mol/L NaCl, 1 mmol/L EDTA, pH 7.5
Flow rate: A: 1 mL/min, B: 0.33 mL/min
Detection: UV @ 260 nm
Temperature: 25 °C
Sample: Hae III-cleaved pBR322 DNA
Sample load: 13 µg in 50 µL

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1007 TSKgel G4000SW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
IM20 Regeneration of TSKgel® SEC-SW HPLC ColumnsInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
SR024 Applications for High-Performance Gel Filtration Columns (TSKgel SW and TSKgel PW columns): Focus on BiopolymersSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
05371TSKgel G2000SW-G4000SW Guard Column for 7.5 mm ID columns10Stainless Steel7.57.5616
05758TSKgel G2000SW-G4000SW Guard Column for 21.5 mm ID columns13Stainless Steel21.57.51381