Separation of Total E. Coli RNA
| Columns: |
TSKgel G4000SW, 13 µm,
7.5 mm ID × 30 cm × 2
TSKgel G4000SW, 17 µm,
7.5 mm ID × 30 cm × 2
|
| Mobile phase: |
0.13 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0, + 1 mmol/L EDTA |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 260 nm |
| Injection vol.: |
5 µg |
| Sample: |
0.1 mL of 1:10 diluted solution of total E. coli RNA:
1. 23s rRNA (1.1 × 106 Da)
2. 16s rRNA (5.6 × 105 Da)
3. 5s rRNA (3.9 × 104 Da)
4. 4s rRNA (2.5 × 104 Da) |
|
|
Double Stranded DNA Fragments
| Column: |
A: TSKgel G3000SW, 10 µm,
7.5 mm ID × 60 cm × 2
B: TSKgel G4000SW, 13 µm,
7.5 mm ID × 60 cm × 2 |
| Mobile phase: |
0.05 mol/L Tris-HCl, 0.2 mol/L NaCl, 1 mmol/L EDTA, pH 7.5 |
| Flow rate: |
A: 1 mL/min, B: 0.33 mL/min |
| Detection: |
UV @ 260 nm |
| Temperature: |
25 °C
|
| Sample: |
Hae III-cleaved pBR322 DNA |
| Sample load: |
13 µg in 50 µL |
|
For additional applications, please see our applications database.