HPLC Columns

TSKgel G4000SWxl

With 8 μm particles and 45 nm pores, TSKgel G4000SWxl columns provide the largest exclusion limit and the widest fractionation range within the TSKgel SWxl column line.  These columns are an excellent choice for pegylated proteins or glycosylated biomolecules.  The fractionation range is applicable for globular protein samples up to 7 million Da.

Widely considered the premium columns for analyzing biopolymers by GFC,  TSKgel G4000SWxl columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.

Also available within the TSKgel G4000SWxl column line is a BioAssist column made of PEEK housing material to reduce sample adsorption to stainless steel or glass.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08542TSKgel G4000SWxl8Stainless Steel7.8301,829

Product Attributes

Base material: Silica
Particle size (mean): 8 µm
Pore size (mean): 45 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 2.0 × 10​4​- 7.0 × 10Da (globular proteins)

Calibration Curves


Column: TSKgel SWXL columns,
7.8 mm ID × 30 cm
Mobile phase: 0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
Detection: UV @ 220 nm
Samples: 1. thyroglobulin (6.6 × 10Da)
2. IgG (1.56 × 10Da)
3. bovine serum albumin
(6.7 ×  10Da)
4. ovalbumin (4.3 × 10Da)
5. peroxidase (4.02 × 10Da)
6. β-lactoglobulin (3.5 × 10Da)
7. myoglobin (1.69 × 10Da)
8. ribonuclease A (1.37 × 10Da)
9. cytochrome C (1.24 × 10Da)
10. glycine tetramer (246 Da)


Separation of φX174 RF DNA-Hae III Digest (4.5 µg/50 µL)


Column: TSKgel G4000SWXL, 8 µm,
7.8 mm ID × 30 cm
Mobile phase: 0.05 mol/L phosphate buffer, pH 7.0 +
0.3 mol/L NaCl + 1 mmol/L EDTA
Flow rate: 0.15 mL/min
Detection: UV @ 260 nm
Temperature: 25 °C

Separation of Immunoglobulins



Column: TSKgel G4000SWXL, 8 µm,
7.8 mm ID × 30 cm
Mobile phase: 20 mmol/L phosphate buffer + 0.3 mol/L NaCl, ph 7.0
Flow rate: 0.5 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 15 µL
Concentration: 0.17 g/L
Samples: 1. human IgM (from myeloma,
MW: 970 kDA)
2. human IgA (colostrum, MW: 390 kDa)
3. human IgG (MW: 150 kDa)
4. transferrin (MW: 80 kDA)
5. human serum albumin (MW: 66 kDa)

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1010 TSKgel G4000SWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
IM20 Regeneration of TSKgel® SEC-SW HPLC ColumnsInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
SR046 TSKgel SWxl Series ColumnsSeparation Report
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08543TSKgel G2000SWxl-G4000SWxl, and QC-PAK GFC Guard Column for 7.8 mm ID columns7Stainless Steel64652