HPLC Columns

TSKgel G5000PWxl

The TSKgel G5000PWxl column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 1 × 106 Da. The smaller particle size of the TSKgel G5000PWxl column provides 1.7× higher resolution than the TSKgel G5000PW column, making the TSKgel G5000PWxl column more suitable for analytical purposes.

The TSKgel G5000PWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008023TSKgel G5000PWxl10Stainless Steel7.8302,341

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 10 µm
Pore size (mean): 100 nm
Calibration range: 4,000 - 8.0 × 10​5​ Da (polyethylene glycols and oxides)



PEG and PEO Calibration Curves


Columns: A. G2500PWXL
B. G3000PWXL
C. G4000PWXL
E. G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: Rl

Protein Calibration Curves


Columns: 1. TSKgel G3000PWXL
2. TSKgel G4000PWXL
3. TSKgel G5000PWXL
4. TSKgel G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.8
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: a. thyroglobulin (6.6 × 10Da)
b. γ-globulin (1.5 × 10Da)
c. albumin (6.7 × 10Da)
d. ovalbumin (4.3 × 10Da)
e. β-lactoglobulin (3.6 × 10Da)
f. myoglobin (1.69 × 10Da)
g. cytochrome C (1.24 × 10Da)


Code #DescriptionLiterature Type
AN125 Molecular Weight Determination of VLPs Using LenS3 Multi-Angle Light Scattering DetectorApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1025 TSKgel G5000PWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR037 Introduction of Aqueous SEC Columns: TSKgel PWxl series Separation Report
SR038 GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008033TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns12Stainless Steel64798