HPLC Columns


TSKgel G-DNA-PW is a specialty column designed for the separation of large polynucleotides such as DNA and RNA fragments of 500-5000 base pairs. TSKgel G-DNA-PW is a smaller particle size version of TSKgel G6000PWxl.

The TSKgel G-DNA-PW column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008032TSKgel G-DNA-PW10Stainless Steel7.8302,341

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 10 µm
Pore size (mean): > 100 nm
Calibration range: 4.0 × 104 - 8.0 × 106 Da (polyethylene glycols and oxides)

Double Stranded DNA Calibration Curves


Columns: TSKgel G-DNA-PW, 10 µm, 
7.8 mm ID × 30 cm × 4
Mobile phase: H2O with 0.3 mol/L NaCl in 0.1 mol/L Tris-HCL, pH 7.5, + 1 mmol/L EDTA
Flow rate: 0.15 mL/min
Detection: UV @ 260 nm
Sample: Eco Rl and Bst Nl-cleaved pBR322 DNA, void volume determined with

Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1027 TSKgel G-DNA-PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR037 Introduction of Aqueous SEC Columns: TSKgel PWxl series Separation Report
SR038 GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008033TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns12Stainless Steel64798