TSKgel GMPWxl

TSKgel GMPWxl is a mixed bed scouting column for water-soluble linear polymers. The pore volume can be accessed by polymers ranging in molecular weight from 500 to 8 × 10⁶ Da.

Mixed bed columns contain a mixture of different pore sizes that have an extended linear calibration range, suitable for samples with a broad molar mass distribution as well as unknown samples. By quickly categorizing the molecular weight profile of an unknown sample, the column enables a fast selection of the best TSKgel PWxl column for routine analysis.

The TSKgel GMPWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008025	TSKgel GMPWxl	13	Stainless Steel	7.8	30	2,341	Inquire

Product Attributes

Base material:	hydroxylated polymethacrylate
Particle size (mean):	13 µm
Pore size (mean):	mixed pore sizes
Calibration range:	1,000 - 8.0 × 10⁶ Da (polyethylene glycols and oxides)

PEG and PEO Calibration Curves

Columns:	A. G2500PWXL B. G3000PWXL C. G4000PWXL D.G5000PWXL E. G6000PWXL F. GMPWXL all 7.8 mm ID × 30 cm
Mobile phase:	distilled H₂O
Flow rate:	1.0 mL/min
Detection:	Rl

Protein Calibration Curves

Columns:	1. TSKgel G3000PWXL 2. TSKgel G4000PWXL 3. TSKgel G5000PWXL 4. TSKgel G6000PWXL 5. TSKgel GMPWXL all 7.8 mm ID × 30 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.8
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Samples:	a. thyroglobulin (6.6 × 10⁵Da) b. γ-globulin (1.5 × 10⁵Da) c. albumin (6.7 × 10⁴Da) d. ovalbumin (4.3 × 10⁴Da) e. β-lactoglobulin (3.6 × 10⁴Da) f. myoglobin (1.69 × 10⁴Da) g. cytochrome C (1.24 × 10⁴Da)

Analysis of Pullulan

Columns:	TSKgel GMPWXL, 13 μm, 7.8 mm ID × 30 cm × 4
Mobile phase:	0.1 mol/L sodium chloride
Flow rate:	1.0 mL/min
Temperature:	40 °C
Detection:	RI LS
Injection vol.:	500 μL
Sample:	1. pullulan P400 2. pullulan P200 3. pullulan P100 4. pullulan P50

Separation of Sodium Polystyrene Sulfonate Standards

Column:	TSKgel GMPWXL, 13 μm, 7.8 mm ID × 30 cm × 4
Mobile phase:	ACN/0.2 mol/L sodium sulfate = 10/90
Flow rate:	1.0 mL/min
Detection:	RI LS
Temperature:	40 °C
Injection vol.:	500 μL
Sample:	sodium poly(styrene sulfonates)

For additional applications, please see our applications database.

Code #	Description	Literature Type
AN031	The Characterization of Sugar Beet Pectin using the EcoSEC GPC System Coupled to Multi-Angle Light Scattering, Quasi-Elastic Light Scattering, and Differential Viscometry	Application Note
AN067	Analysis of Hyaluronic Acid using the EcoSEC GPC System coupled to Differential Viscometry	Application Note
AN125	Molecular Weight Determination of VLPs Using LenS3 Multi-Angle Light Scattering Detector	Application Note
AN133	Monitoring Structural Changes in Polysaccharides using SEC-MALS	Application Note
AN138	SEC-MALS Sensitively Quantifies Adeno-Associated Viruses and Detects Impurities	Application Note
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
DS1028	TSKgel GMPWxl Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01B	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series Columns	Product Catalog
SR037	Introduction of Aqueous SEC Columns: TSKgel PWxl series	Separation Report
SR038	GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008033	TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns	12	Stainless Steel	6	4	798