HPLC Columns


TSKgel GMPWxl is a mixed bed scouting column for water-soluble linear polymers. The pore volume can be accessed by polymers ranging in molecular weight from 500 to 8 × 106  Da.


Mixed bed columns contain a mixture of different pore sizes that have an extended linear calibration range, suitable for samples with a broad molar mass distribution as well as unknown samples. By quickly categorizing the molecular weight profile of an unknown sample, the column enables a fast selection of the best TSKgel PWxl column for routine analysis. 


The TSKgel GMPWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008025TSKgel GMPWxl13Stainless Steel7.8302,341

Product Attributes

Base material: hydroxylated polymethacrylate
Particle size (mean): 13 µm
Pore size (mean): mixed pore sizes
Calibration range: 1,000 - 8.0 × 106​ Da (polyethylene glycols and oxides)




PEG and PEO Calibration Curves


Columns: A. G2500PWXL
B. G3000PWXL
C. G4000PWXL
E. G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: distilled H2O
Flow rate: 1.0 mL/min
Detection: Rl

Protein Calibration Curves


Columns: 1. TSKgel G3000PWXL
2. TSKgel G4000PWXL
3. TSKgel G5000PWXL
4. TSKgel G6000PWXL
all 7.8 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.8
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: a. thyroglobulin (6.6 × 10Da)
b. γ-globulin (1.5 × 10Da)
c. albumin (6.7 × 10Da)
d. ovalbumin (4.3 × 10Da)
e. β-lactoglobulin (3.6 × 10Da)
f. myoglobin (1.69 × 10Da)
g. cytochrome C (1.24 × 10Da)



Analysis of Pullulan




Columns: TSKgel GMPWXL, 13 μm,
7.8 mm ID × 30 cm × 4
Mobile phase: 0.1 mol/L sodium chloride 
Flow rate: 1.0 mL/min
Temperature: 40 °C
Detection: RI
Injection vol.: 500 μL
Sample: 1. pullulan P400
2. pullulan P200
3. pullulan P100
4. pullulan P50


Separation of Sodium Polystyrene Sulfonate Standards


Column: TSKgel GMPWXL, 13 μm,
7.8 mm ID × 30 cm × 4

Mobile phase: ACN/0.2 mol/L sodium sulfate = 10/90
Flow rate: 1.0 mL/min
Detection: RI
Temperature: 40 °C
Injection vol.: 500 μL
Sample: sodium poly(styrene sulfonates)

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AN031 The Characterization of Sugar Beet Pectin using the EcoSEC GPC System Coupled to Multi-Angle Light Scattering, Quasi-Elastic Light Scattering, and Differential ViscometryApplication Note
AN067 Analysis of Hyaluronic Acid using the EcoSEC GPC System coupled to Differential ViscometryApplication Note
AN125 Molecular Weight Determination of VLPs Using LenS3 Multi-Angle Light Scattering DetectorApplication Note
AN133 Monitoring Structural Changes in Polysaccharides using SEC-MALSApplication Note
AN138 SEC-MALS Sensitively Quantifies Adeno-Associated Viruses and Detects ImpuritiesApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1028 TSKgel GMPWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
SR037 Introduction of Aqueous SEC Columns: TSKgel PWxl series Separation Report
SR038 GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0008033TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns12Stainless Steel64798