HPLC Columns

TSKgel SuperAW2500

The TSKgel SuperAW2500 column is packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in polar organic solvents (methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP).  This column is stable in a wide variety of organic solvents at concentrations up to 100%.

The main application area for the TSKgel SuperAW2500 column is the analyses of polymers that are soluble in polar organic solvents up to 2,000 Da. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in 10 mmol/L LiBr in DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in hexafluoroisopropanol (HFIP). Reductions in analysis time and mobile phase consumption due to the small column dimensions (6 mm ID x 15 cm) make the TSKgel SuperAW2500 column ideal for high throughput applications.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0019315TSKgel SuperAW25004Stainless Steel6152,053

Product Attributes

Matrix: hydrophilic methacrylate
Particle size (mean): 4 µm
Pore size (mean): 2.5 nm
Exclusion limits
    PEO in H2O: 5,000
    PS in DMF with
    10 mmol/L LiBr:
1 × 104
    PEG in MeOH with
    10 mmol/L LiBr:
1 × 104




Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves


Columns: TSKgel SuperAW columns,
6.0 mm ID × 15 cm
Mobile phase: A. H2O
B. MeOH with 10 mmol/L LiBr
C. DMF with 10 mmol/L LiBr
Flow rate: 0.6 mL/min
Detection: RI
Temperature: 25°C
Samples: A. polyethylene oxide (PEO), 
B. polyethylene glycol (PEG),and
C. ethylene glycol (EG) standards


Analysis of a Surfactant



Column: TSKgel SuperAW2500, 7 μm, 6.0 mm ID × 15 cm 
Mobile phase: ACN, ACN Solution
Flow rate: 0.6 mL/min
Detection: UV @ 280 nm
Temperature: 40 °C
Injection vol.: 20 μL


Analysis of Medicated Cream



Column: TSKgel SuperAW2500, 7 μm, 6.0 mm ID × 15 cm × 2
Mobile phase: ethanol
Flow rate: 0.6 mL/min
Detection: UV @ 275 nm
Temperature: 40 °C
Injection vol.: 10 μL

For additional applications, please see our applications database.



Column Efficiency of TSKgel SuperAW Columns





Column: TSKgel SuperAW columns,
6.0 mm ID × 15 cm
Mobile phase: H2O
Flow rate: 0.6 mL/min
Detection: RI
Temperature: 25 °C
Injection vol.: 5 µL (2.5 g/L)
Sample: ethylene glycol

Code #DescriptionLiterature Type
AN005 High-Throughput SEC of Water Soluble Polymers Using TSKgel SuperAW ColumnsApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1186 TSKgel SuperAW2500 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01C TSKgel U/HPLC Columns 2019 Product Guide -- SEC, Alpha and SuperAW ColumnsProduct Catalog
PO07 TSKgel SuperAW ColumnsProduct Overview
SR099 TSKgel SuperAW Series High-performance SEC Semi-Micro ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0019321TSKgel SuperAW2500-4000 Guard Column for 6.0 mm ID columns7Stainless Steel4.63.5693