TSKgel SuperH2500

The TSKgel SuperH2500 column is packed with rigid, porous, polymer beads. It is for the non-aqueous separation of small molecules and oligomers less than 20,000 Da. The maximum operating temperature for the TSKgel SuperH2500 column is 140 °C. This column is shipped in THF, which can be exchanged for a wide variety of organic solvents.

This TSKgel SuperH2500 column is a high efficiency/high throughput version of the conventional TSKgel G2500Hhr column. Both column types are based on the same bead chemistry.

The volume of the 6 mm ID × 15 cm TSKgel SuperH2500 column is 3.4 times smaller than that of a conventional 7.8 mm ID × 30 cm column. As a result, peak volumes will be proportionally smaller on this column compared to a corresponding TSKgel Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0017992	TSKgel SuperH2500	3	Stainless Steel	6	15	2,053	Inquire

Product Attributes

Matrix:	polystyrene-divinylbenzene
Particle size (mean):	3 µm
Pore size (mean):	3 nm
Exclusion limit:	2.0 × 10⁴ Da

Polystyrene Calibration Curves

Columns:	TSKgel SuperH columns, 6.0 mm ID × 15 cm
Mobile phase:	THF
Flow rate:	0.6 mL/min
Detection:	UV @ 254 nm
Temperature:	25 °C
Samples:	polystyrene standards

Separation of Standard Polystyrenes using a TSKgel SuperH2500 Column

Columns:	TSKgel SuperH2500, 3 µm, 6 mm ID × 15 cm
Mobile phase:	THF, chloroform, DMF, carbon tetrachloride
Flow rate:	0.6 mL/min
Temperature:	25°C
Detection:	UV/VIS @ 254 nm or 270 nm
Samples:	1) polystyrene (1.9 × 10⁵ Da) 2) polystyrene (9.1 × 10⁴ Da) 3) polystyrene (2,800 Da) 4) polystyrene A-500

For additional applications, please see our applications database.

Dependence of Separation Performance on Band Spreading in Detector

Column:	TSKgel SuperH2500, 3 µm, 6mm ID × 15 cm × 2
Mobile phase:	THF
Flow rate:	0.6 mL/min
Detection:	UV @ 254 nm
Temperature:	25 °C
Samples:	1. standard polystyrene A-500 (0.1%), 10 µL 2. Epikote 1004 (0.1%), 10 µL

Code #	Description	Literature Type
AN108	Determination of the molecular weight distribution of phenol-formaldehyde resins using GPC	Application Note
DS1192	TSKgel SuperH2500 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01D	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series Columns	Product Catalog
SR088	High Speed and High Performance Semi-micro GPC Columns: TSKgel SuperH Series	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018002	TSKgel SuperH1000-SuperH4000 Guard Column for 6 mm ID columns	3	Stainless Steel	4.6	3.5	692