HPLC Columns

TSKgel SuperH2500

The TSKgel SuperH2500 column is packed with rigid, porous, polymer beads. It is for the non-aqueous separation of small molecules and oligomers less than 20,000 Da. The maximum operating temperature for the TSKgel SuperH2500 column is 140 °C. This column is shipped in THF, which can be exchanged for a wide variety of organic solvents.

This TSKgel SuperH2500 column is a high efficiency/high throughput version of the conventional TSKgel G2500Hhr column. Both column types are based on the same bead chemistry.
The volume of the 6 mm ID × 15 cm TSKgel SuperH2500 column is 3.4 times smaller than that of a conventional 7.8 mm ID × 30 cm column. As a result, peak volumes will be proportionally smaller on this column compared to a corresponding TSKgel Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0017992TSKgel SuperH25003Stainless Steel6152,053

Product Attributes

Matrix: polystyrene-divinylbenzene
Particle size (mean): 3 µm
Pore size (mean): 3 nm
Exclusion limit: 2.0 × 104​ Da

Polystyrene Calibration Curves


Columns: TSKgel SuperH columns,
6.0 mm ID × 15 cm
Mobile phase: THF
Flow rate: 0.6 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: polystyrene standards


Separation of Standard Polystyrenes using a TSKgel SuperH2500 Column


Columns: TSKgel SuperH2500, 3 µm, 
6 mm ID × 15 cm

Mobile phase: THF, chloroform, DMF, carbon tetrachloride
Flow rate: 0.6 mL/min
Temperature: 25°C
Detection: UV/VIS @ 254 nm or 270 nm
Samples: 1) polystyrene (1.9 × 105 Da)
2) polystyrene (9.1 × 104 Da)
3) polystyrene (2,800 Da)
4) polystyrene A-500

For additional applications, please see our applications database.



Dependence of Separation Performance on Band Spreading in Detector 



TSKgel SuperH2500, 3 µm,
6mm ID × 15 cm × 2

Mobile phase: THF
Flow rate: 0.6 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: 1. standard polystyrene A-500 (0.1%), 10 µL
2. Epikote 1004 (0.1%), 10 µL
Code #DescriptionLiterature Type
AN108 Determination of the molecular weight distribution of phenol-formaldehyde resins using GPCApplication Note
DS1192 TSKgel SuperH2500 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01D TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series ColumnsProduct Catalog
SR088 High Speed and High Performance Semi-micro GPC Columns: TSKgel SuperH SeriesSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018002TSKgel SuperH1000-SuperH4000 Guard Column for 6 mm ID columns3Stainless Steel4.63.5692