HPLC Columns

TSKgel SuperH3000

The TSKgel SuperH3000 column is packed with rigid, porous, polymer beads. It is for the non-aqueous separation of small molecules and oligomers less than 60,000 Da. The maximum operating temperature for the TSKgel SuperH3000 column is 140 °C. This column is shipped in THF, which can be exchanged for a wide variety of organic solvents.

This TSKgel SuperH3000 column is a high efficiency/high throughput version of the conventional TSKgel G3000Hhr column. Both column types are based on the same bead chemistry.
The volume of the 6 mm ID × 15 cm TSKgel SuperH3000 column is 3.4 times smaller than that of a conventional 7.8 mm ID × 30 cm column. As a result, peak volumes will be proportionally smaller on this column compared to a corresponding TSKgel Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0017993TSKgel SuperH30003Stainless Steel6152,053

Product Attributes

Matrix: polystyrene-divinylbenzene
Particle size (mean): 3 µm
Pore size (mean): 7.5 nm
Exclusion limit: 6.0 × 104​ Da

Polystyrene Calibration Curves


Columns: TSKgel SuperH columns,
6.0 mm ID × 15 cm
Mobile phase: THF
Flow rate: 0.6 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: polystyrene standards


Temperature Dependence of Separation on Epoxy Resin



TSKgel SuperH3000, 3µm,
6mm ID × 15 cm × 2 +
TSKgel SuperH2500, 3µm,
6mm ID × 15 cm × 3

Mobile phase: THF
Flow rate: 0.6 mL/min
Detection: UV @ 254 nm
Samples: Epikote 1004 (0.1%),10 µL
Code #DescriptionLiterature Type
AN035 Molar Mass Measurements of an Isocyanate Modified Polyurethane Prepolymer in Less than 1 Hour with the EcoSEC GPC System Application Note
AN036 EcoSEC GPC Workstation Software Multiprocessing FeatureApplication Note
AN038 Synthesis Monitoring and Oligomeric Analysis of PEGylated PolymersApplication Note
AN052 Photodegradable Polymer Degradation Analysis Using the EcoSEC GPC SystemApplication Note
DS1193 TSKgel SuperH3000 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01D TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series ColumnsProduct Catalog
SR088 High Speed and High Performance Semi-micro GPC Columns: TSKgel SuperH SeriesSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018002TSKgel SuperH1000-SuperH4000 Guard Column for 6 mm ID columns3Stainless Steel4.63.5692