HPLC Columns

TSKgel SuperHM-H

The TSKgel SuperHM-H mixed bed column is packed with rigid, multiple pore size, polymer beads that are blended to provide an extended linear calibration curve. Mixed bed columns are commonly used for unknown samples and for the analysis of polymers with a broad molecular weight distribution. This column provides high resolution of polymers with molecular weights of less than 4 × 108 Da.

The maximum operating temperature for the TSKgel SuperHM-H column is 140 °C. This column is shipped in THF, which can be exchanged for a wide variety of organic solvents.

The TSKgel SuperHM-H column is a high efficiency/high throughput version of the conventional TSKgel GMHhr-H column. Both column types are based on the same bead chemistry.
The volume of the 6 mm ID × 15 cm TSKgel SuperHM-H column is 3.4 times smaller than that of a conventional 7.8 mm ID × 30 cm column. As a result, peak volumes will be proportionally smaller on this column compared to a corresponding TSKgel Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018001TSKgel SuperHM-H3Stainless Steel6152,240

Product Attributes

Matrix: polystyrene-divinylbenzene
Particle size (mean): 3 µm
Pore size (mean): mixed
Exclusion limit: 4.0 × 10​Da

Polystyrene Calibration Curves


Columns: TSKgel SuperH columns,
6.0 mm ID × 15 cm
Mobile phase: THF
Flow rate: 0.6 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: polystyrene standards


Separation of Standard Polystyrenes using a TSKgel SuperHM-H Column


Columns: TSKgel SuperHM-H, 3 µm,
6 mm ID × 15 cm

Mobile phase: THF, chloroform, DMF, carbon tetrachloride
Flow rate: 0.6 mL/min
Temperature: 25°C
Detection: UV/VIS @ 254 nm
Samples: 1) polystyrene (2.89 × 10Da)
2) polystyrene (4.22 × 10Da)
3) polystyrene (1.07 × 105 Da)
4) polystyrene (1.67 × 104 Da)
5) polystyrene (2,800 Da)

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
DS1234 TSKgel SuperHM-type ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01D TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series ColumnsProduct Catalog
SR088 High Speed and High Performance Semi-micro GPC Columns: TSKgel SuperH SeriesSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018003TSKgel SuperH5000-7000;HM-L;-N;-M;-H Guard Column for 6 mm ID columns3Stainless Steel4.63.5693