HPLC Columns

TSKgel SuperHZ4000

TSKgel SuperHZ4000 columns are packed with rigid, porous, 3 μm polymer beads. These ultra-fine particles provide high resolution for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 4 × 105 Da. The maximum operating temperature for the TSKgel SuperHZ4000 columns are 80 °C.  The THF shipping solvent may be replaced by a limited number of organic solvents.
TSKgel SuperHZ4000 columns are ideal for high throughput, high efficiency GPC applications such as those encountered in combinatorial chemistry experiments. These columns feature ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers.
TSKgel SuperHZ4000 column dimensions are 6 mm ID × 15 cm and 4.6 mm ID × 15 cm versus 7.8 mm ID × 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6 mm ID) and a factor of 5.8 (4.6 mm ID) versus the same component eluting from a corresponding TSKgel G4000Hxl column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19313TSKgel SuperHZ40003Stainless Steel4.6151,771
19306TSKgel SuperHZ40003Stainless Steel6151,692

Product Attributes

Matrix: polystyrene-divinylbenzene
Particle size (mean): 3 µm
Pore size (mean): 20 nm
Exclusion limit: 4.0 × 10Da
Max. temperature: 80 °C

Polystyrene Calibration Curves


Columns: TSKgel SuperHZcolumns,
4.6 mm ID × 15 cm
Mobile phase: THF
Flow rate: 0.35 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Injection vol.: 2 µL
Samples: polystyrene standards


Comparison of Analysis on TSKgel SuperHZ and TSKgel HXL Columns



Columns: A: TSKgel SuperHZ columns
(4000, 3000, 2500),
4.6 mm ID × 15 cm ×3
B:TSKgel HXL columns
(4000, 3000, 2500),
7.8 mm ID ×30 cm ×3
Mobile phase: THF
Flow rate: A. 0.35 mL/min  B. 1.0 mL/min
Detection: RI
Temperature: 40 °C
Injection vol.: A. 5 µL  B. 30 µL
Samples: phenolic resin

Code #DescriptionLiterature Type
AN006 High-Throughput GPC Analysis of Phenolic Resin Using TSKgel SuperHZ ColumnsApplication Note
AN054 Analysis of Polymers for Therapeutics Using the EcoSEC GPC System Application Note
AN080 Analysis of Asphalt Binders using Gel Permeation Chromatography Application Note
DS1198 TSKgel SuperHZ4000 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01D TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series ColumnsProduct Catalog
SR101 SuperHZ Series Ultra-High Throughput GPC ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19314TSKgel SuperHZ1000-4000 and HZM-N and -M Guard Column for 4.6 mm ID columns3Stainless Steel4.62570
19666TSKgel SuperHZ1000-4000 and HZM-N and -M Guard Column for 6 mm ID columns3Stainless Steel4.63.5570