HPLC Columns


TSKgel DEAE-2SW columns are packed with 5 µm porous spherical silica beads which are surface modified with a weak anion exchange group. They are used for the separation and analysis of nucleotides, nucleosides, peptides, small proteins and other biologically active molecules. A 2 mm ID column is available for applications that are sample limited.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018761TSKgel DEAE-2SW5Stainless Steel2251,419
0007168TSKgel DEAE-2SW5Stainless Steel4.6251,704

Product Attributes

Matrix: Silica
Particle size (mean): 5 µm
Pore size (mean): 12.5 nm
Functional group: CH2CH2N+(C2H5)3
Counter ion: H2PO4-
pH stability: 2.0 - 7.5
Small ion capacity: >0.3 eq/L
pKa: 11.2






Separation of Nucleotides



Column: TSKgel DEAE-2SW, 5 µm,
4.6 mm ID × 25 cm

Mobile phase: A: CH3CN in 0.1 mol/L phosphate, pH 3.0, 20/80
B: CH3CN in 0.5 mol/L phosphate, pH 3.0, 20/80
Gradient: 30 min linear gradient from buffer A to B
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Samples: 1. AMP 2. IMP 3. GMP 4. ADP 5. ATP


Separation of Phosphorothioates



Column: TSKgel DEAE-2SW, 5 µm,
4.6 mm ID × 25 cm

Mobile phase: A: 50 mmol/L ammonium acetate
B: 1.5 mol/L ammonium acetate
Gradient: linear, 0-100% B in 60 minutes
Flow rate: 1 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: 1. 2 base phosphorothioate oligonucleotide
2. 3 base phosphorothioate oligonucleotide
3. 4 base phosphorothioate oligonucleotide
4. 5 base phosphorothioate oligonucleotide


For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO02 Purification of Nucleic Acids Application Overview
DS1066 TSKgel DEAE-2SW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog
SR097 Application of TSKgel Semi-Micro for High-Sensitivity Analyses Separation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0007648TSKgel DEAE-2SW and -3SW Guardgel Kit for 4.6 mm ID and 7.5 mm ID columns0Stainless Steel 0913
0019308TSKgel Guard Cartridge Holder for 2 mm ID cartridges0 21506
0042154TSKgel DEAE-2SW Guard Cartridge for 2 mm ID column5Stainless Steel210