Analysis of High MM RNA
![IEX_DEAE-5PW-Fig8.png](https://separations.us.tosohbioscience.com/Image Library/TBL/Product Catalog/HPLC Columns/Anion Exchange/IEX_DEAE-5PW-Fig8.png)
Columns: |
TSKgel DEAE-5PW, 10 µm,
6 mm ID × 15 cm (custom) |
Mobile phase: |
300 min linear gradient from 0.3 mol/L to 1.0 mol/L NaCl in 0.1 mol/L Tris-HCl, pH 7.6 |
Flow rate: |
1.0 mL/min |
Detection: |
UV @ 260 nm |
Sample: |
total E. coli RNA |
Detection of HIV-1 PCR-Amplified 130 bp Target
![IEX_DEAE-5PW-Fig9.png](https://separations.us.tosohbioscience.com/Image%20Library/TBL/Product%20Catalog/HPLC%20Columns/Anion%20Exchange/IEX_DEAE-5PW-Fig9.png)
Column: |
TSKgel DEAE-5PW, 10 µm,
7.5 mm ID × 7.5 cm
|
Mobile phase: |
A: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0
B: 25 mmol/L Tris-HCl, 1 mmol/L EDTA, pH 8.0, with 1 mol/L NaCl |
Gradient: |
25-60% B in 30 minutes |
Flow rate: |
1.0 mL/min |
Detection: |
UV @ 260 nm |
Temperature: |
25 °C
|
Sample: |
pBR322 plasmid |
Sample load: |
2.5 mg in 1 mL |
|
|
Separation of Monoclonal Antibody
![IEX_DEAE-5PW-Fig12.png](https://separations.us.tosohbioscience.com/Image Library/TBL/Product Catalog/HPLC Columns/Anion Exchange/IEX_DEAE-5PW-Fig12.png)
Column: |
TSKgel DEAE-5PW, 10 µm,
7.5 mm ID x 7.5 cm
|
Mobile phase: |
A: 20 mmol/L Tris-HCl, pH 8.5
B: A + 0.5 mol/L NaCl
A to B linear gradient (60 min) |
Flow rate: |
1.0 mL/min |
Detection: |
UV @ 280 nm |
Temperature: |
25 °C
|
Sample: |
anti-human albumin (IgG1), diluted solution of mouse ascites (168 µg in 40 µL) |
|
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