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HPLC Columns

TSKgel DEAE-NPR

The TSKgel DEAE-NPR column is packed with spherical 2.5 µm, non-porous, high efficiency, hydrophilic polymer beads which are surface modified with a weak anion exchange group. These columns are used for the high speed separation and analysis of proteins and poly- and oligonucleotides. TSKgel DEAE-NPR columns are particularly useful for high resolution separation of DNA digests or fragments.

NPR stands for Non-Porous Resin.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
13075TSKgel DEAE-NPR2.5Stainless Steel4.63.51,205

          

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 2.5 µm
Pore size (mean): non-porous
Functional group: CH2CH2N+(C2H5)3
Counter ion: Cl-
pH stability: 2.0 - 12.0
Capacity (mg BSA/mL): 5
Small ion capacity: >0.1 eq/L
pKa: 11.2

 

 

 

 

                 

Analysis of DNA Digest

IEX_DEAE-NPR-Fig14.png

 

Columns: TSKgel DEAE-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm, with guard column, 4.6 mm ID × 0.5 cm

Mobile phase: A: 0.02 mol/L Tris-HCl, pH 9.0
B: mobile phase A plus 1.0 mol/L NaCl
Gradient: 15 min linear gradient from 48% to 65% mobile phase B
Flow rate: 1.5 mL/min
Detection: UV @ 260 nm
Pressure: 14 MPa
Temperature: 40 °C
Sample: Hae III digest of pBR322 DNA, (base pair number for each peak is indicated)

 

Detection of HIC-1 PCR-Amplified 130 bp Target

IEX_DEAE-NPR-Fig17.png

 

Column: TSKgel DEAE-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm

Mobile phase: A: 20 mmol/L Tris-HCl with 0.25 mol/L NaCl, pH 7.7
B: 20 mmol/L Tris-HCl with 1 mol/L NaCl, pH 7.7
Flow rate: 1 mL/min
Detection: [email protected] 260 nm
Temperature: ambient 
Sample: HIV-1 PCR-amplified 130 bp target
Sample load: 20 µL

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AO02 Purification of Nucleic Acids Application Overview
DS1031 TSKgel DEAE-NPR ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
TP133 High Speed and High Resolution Anion Exchange Chromatography for Biological Samples on Non-Porous PackingsTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
17088TSKgel DEAE-NPR Guard Column for 4.6 mm ID column5Stainless Steel4.60.5412