Analysis of DNA Digest
| Columns: |
TSKgel DEAE-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm, with guard column, 4.6 mm ID × 0.5 cm
|
| Mobile phase: |
A: 0.02 mol/L Tris-HCl, pH 9.0
B: mobile phase A plus 1.0 mol/L NaCl |
| Gradient: |
15 min linear gradient from 48% to 65% mobile phase B |
| Flow rate: |
1.5 mL/min |
| Detection: |
UV @ 260 nm |
| Pressure: |
14 MPa |
| Temperature: |
40 °C
|
| Sample: |
Hae III digest of pBR322 DNA, (base pair number for each peak is indicated) |
|
|
Detection of HIC-1 PCR-Amplified 130 bp Target
| Column: |
TSKgel DEAE-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm
|
| Mobile phase: |
A: 20 mmol/L Tris-HCl with 0.25 mol/L NaCl, pH 7.7
B: 20 mmol/L Tris-HCl with 1 mol/L NaCl, pH 7.7 |
| Flow rate: |
1 mL/min |
| Detection: |
UV@ 260 nm |
| Temperature: |
ambient
|
| Sample: |
HIV-1 PCR-amplified 130 bp target
|
| Sample load: |
20 µL
|
|
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