Plasmid Analysis
| Columns: |
TSKgel DNA-NPR, 2.5 µm,
4.6 mm ID × 7.5 cm
|
| Mobile phase: |
A: 20 mmol/L Tris, pH 9.0
B: 20 mmol/L Tris, pH 9.0 with 1 mol/L NaCl |
| Gradient: |
linear gradient from 50% to 65% B in 10 column volumes |
| Flow rate: |
1 mL/min |
| Detection: |
UV @ 260 nm |
| Sample: |
PUC19 plasmid |
|
|
Oligonucleotides
| Column: |
TSKgel DNA-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm
|
| Mobile phase: |
A: 10 mmol/L sodium bromide, 20 mmol/L NaOH, pH 12, 1% diethylamine
B: 1 mol/L sodium bromide, 20 mmol/L NaOH, pH 12, 1% diethylamine |
| Gradient: |
3.5 min (20% B) 12 min (20% B) 45 min (55% B) |
| Flow rate: |
1.0 mL/min |
| Temperatures: |
60 °C (column), 4 °C (sample chamber) |
| Sample: |
crude deprotected 13-mer oligonucleotide |
|
For additional applications, please see our applications database.