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HPLC Columns

TSKgel DNA-NPR

The TSKgel DNA-NPR column is packed with 2.5 µm, non-porous, high efficiency, hydrophilic polymer beads which are surface modified with a weak anion exchange group. Because this column contains non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality, whereas protein recovery is generally very high.

The TSKgel DNA-NPR column dimensions are optimized for the high efficiency separation of DNA fragments, PCR products, or plasmids.

NPR stands for Non-Porous Resin.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18249TSKgel DNA-NPR2.5Stainless Steel4.67.51,639

           

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 2.5 µm
Pore size (mean): non-porous
Functional group: proprietary
Counter ion: ClO4-
pH stability: 2.0 - 12.0
Capacity (mg BSA/mL): 5
Small ion capacity: >0.1 eq/L
pKa: 11.2

 

 

 

 

                  

Plasmid Analysis

IEX_DNA-NPR-Fig15_rev.png   

 

Columns: TSKgel DNA-NPR,  2.5 µm,
4.6 mm ID × 7.5 cm

Mobile phase: A: 20 mmol/L Tris, pH 9.0
B: 20 mmol/L Tris, pH 9.0 with 1 mol/L NaCl
Gradient: linear gradient from 50% to 65% B in 10 column volumes
Flow rate: 1 mL/min
Detection: UV @ 260 nm
Sample: PUC19 plasmid

 

Oligonucleotides

IEX_DNA-NPR-Fig16.png

 

Column: TSKgel DNA-NPR,  2.5 µm,
4.6 mm ID × 3.5 cm

Mobile phase: A: 10 mmol/L sodium bromide, 20 mmol/L NaOH, pH 12, 1% diethylamine
B: 1 mol/L sodium bromide, 20 mmol/L NaOH, pH 12, 1% diethylamine
Gradient: 3.5 min (20% B) 12 min (20% B) 45 min (55% B)
Flow rate: 1.0 mL/min
Temperatures: 60 °C (column), 4 °C (sample chamber)
Sample: crude deprotected 13-mer oligonucleotide

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN014 Purification of a 13-mer DNA Phosphorothioated Crude Deprotected Oligonucleotide by Strong Anion Exchange Chromatography Using TSKgel DNA-NPRApplication Note
AN121 Rapid Analysis of Plasmid Topoisomers by Anion Exchange ChromatographyApplication Note
AO02 Purification of Nucleic Acids Application Overview
DS1128 TSKgel DNA-NPR ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog
PO12 TSKgel DNA-NPR Anion Exchange ColumnsProduct Overview
TP133 High Speed and High Resolution Anion Exchange Chromatography for Biological Samples on Non-Porous PackingsTechnical Presentation
TP169 Selectivity studies in the analytical separation of oligonucleotides using anion exchange chromatographyTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18253TSKgel DNA-NPR Guard Column for 4.6 mm ID column5Stainless Steel4.60.5412