Products

HPLC Columns

TSKgel DNA-STAT

The TSKgel DNA-STAT column is packed with 5 µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered quaternary ammonium anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.

Applications for the TSKgel DNA-STAT columns include the separation of DNA fragments, nucleic acids and nucleotides.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
21962TSKgel DNA-STAT5Stainless Steel4.6101,192
            

Product Attributes

Matrix: hydrophilic polymer
Particle size (mean): 5 µm
Pore size (mean): nonporous
Functional group: quaternary ammonium
Counter ion: Cl-
pH stability: 3.0 - 10.0
Static binding capacity (mg BSA/g dry gel):

ca. 35 (5 µm)

Small ion capacity: 270 µeq/g dry gel
pKa: 10.5

 

 

 

 

                   

Separation of Large DNA Fragments

IEX_DNA-STAT-Fig19.png

 

Columns: TSKgel DNA-STAT, 5 µm,
4.6 mm ID × 10 cm

Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.5
B: 0.75 mol/L NaCl in buffer A
Gradient: 0 min (50% B) 25 min (75% B)
Flow rate: 0.8 mL/min
Detection: UV @ 260 nm

 

Separation of Large DNA Fragments Containing more than 10kb

IEX_DNA-STAT_fig1new.png

 

Columns: TSKgel DNA-STAT, 5 µm,
4.6 mm ID × 10 cm

Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.5
B: 1.0 mol/L NaCl, pH 8.5
Gradient: B) 75 to 100% (20 min.)
Flow rate: 0.5 mL/min
Detection: UV @ 260 nm
Sample: 1 kb Ladder

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
DS1232 TSKgel STAT Series Anion Exchange ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog
PO23 TSKgel Q-STAT and TSKgel DNA-STAT columnsProduct Overview
SR109 TSKgel STAT Columns for High Performance Ion Exchange Chromatography Separation Report
TP133 High Speed and High Resolution Anion Exchange Chromatography for Biological Samples on Non-Porous PackingsTechnical Presentation
TP169 Selectivity studies in the analytical separation of oligonucleotides using anion exchange chromatographyTechnical Presentation