Products

HPLC Columns

TSKgel Q-STAT

TSKgel Q-STAT columns are packed with 7 and 10 µm non-porous resin particles of which the surface consists of long hydrophilic chains of open access network of multi-layered quaternary ammonium anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure, making these columns suitable for all HPLC and FPLC systems.

Applications for the TSKgel Q-STAT columns include the separation of proteins, peptides, low molecular weight nucleic acids, aggregates and charge isomers of monoclonal antibodies, PEGylated proteins, oligo DNA, and siRNA.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
21961TSKgel Q-STAT7Stainless Steel4.6101,192
21960TSKgel Q-STAT10Stainless Steel33.51,127
           

Product Attributes

Matrix: hydrophilic polymer
Particle size (mean): 7 µm and 10 µm
Pore size (mean): nonporous
Functional group: quaternary ammonium
Counter ion: Cl-
pH stability: 3.0 - 10.0
Static binding capacity (mg BSA/g dry gel):

ca. 25 (7 µm)

ca. 20 (10 µm)

Small ion capacity: 270 µeq/g dry gel
pKa: 10.5

 

 

 

 

                  

Separation of Mouse Ascites Fluid Containing Monoclonal Antibodies and purified Monoclonal Antibodies

IEX_Q-STAT-Fig20.png

 

Columns: TSKgel Q-STAT, 7 µm,
4.6 mm ID × 10 cm
Mobile phase: A: 20 mmol/L Tris-HCl buffer, pH 8.5
B: 0.5 mol/L NaCl in 20 mmol/L Tris-HCl buffer, pH 8.5
Gradient: A to B linear gradient (10 min)
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Sample: Top: 1/10 dilution of mouse ascites containing mAb
Bottom: purified mouse mAb
Sample diluted 10-fold with eluent A

 

 

Separation of Proteins Within 1 Minute

IEX_Q-STAT_fig1.png

 

Column: A. TSKgel Q-STAT, 10 µm,
3 mm ID × 3.5 cm
B. ProSwift WAX-1S Monolith
4 mm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.5
B: 0.5 mol/L NaCl in buffer A
Gradient: 0 min (0% B), 1 min (100% B)
Flow rate: 2.0 mL/min
Detection: UV @ 280 nm
Samples: 1. conalbumin
2. ovalbumin
3. trypsin inhibitor

Analysis of IgG

IEX_Q-STAT-Fig21.png

 

Column: A: TSKgel Q-STAT, 7 µm,
4.6 mm ID × 10 cm
B: ProPac® WAX-10, 10 µm, 4 mm ID × 25 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.5
B: 0.5 mol/L NaCl in buffer A
Gradient: 0 min (0% B) 10 min (100% B)
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Sample: pepsin digested mAb

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN039 Separation of mAb Isoforms Using Controlled pH Gradients and Ion Exchange Chromatography ColumnsApplication Note
AN104 An IE-UHPLC Method for Testing of Long-Term Alcohol AbuseApplication Note
DS1232 TSKgel STAT Series Anion Exchange ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog
PO23 TSKgel Q-STAT and TSKgel DNA-STAT columnsProduct Overview
SR109 TSKgel STAT Columns for High Performance Ion Exchange Chromatography Separation Report
TP133 High Speed and High Resolution Anion Exchange Chromatography for Biological Samples on Non-Porous PackingsTechnical Presentation
TP170 Separation of mAb isoforms using controlled pH gradients and AEX/CEX columns packed with 7 and 10 μm hydrophilic non-porous resin particlesTechnical Presentation
TP199 Separation of Sialyated Glycoprotein Isoforms using Strong Anion Exchange (SAX) Chromatography with Controlled pH GradientsTechnical Presentation