Products

HPLC Columns

TSKgel Sugar AXG

The TSKgel Sugar AXG column is packed with 10 µm porous spherical polymer beads which are surface modified with a strong anion exchange group. They are used for the gradient separation and analysis of monosaccharides, disaccharides, and sugar alcohols.

For isocratic separation of carbohydrates where lower and constant back pressures may be generated, please see the TSKgel Sugar AXI column, which is packed with 8 µm particles.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08640TSKgel Sugar AXG10Stainless Steel4.6151,431
            

Product Attributes

Matrix: PS-DVB polymer
Particle size (mean): 10 µm
Pore size (mean): 6 nm
Functional group: trimethylamino
Counter ion: HBO3-
pH stability: 1.0 - 14.0
Small ion capacity:

>1.2 eq/L 

pKa: 12.5

 

 

 

 

                 

Separation of Saccharide Mixture

IEX_Sugar-AXG-Fig22_rev.png

Columns: TSKgel Sugar AXG, 10 µm,
4.6 mm ID × 15 cm

Mobile phase: step gradient: 6 min buffer A, 0.6 mol/L boric acid, pH 7.7; then 27 min buffer B, 0.7 mol/L boric acid, pH 7.25; then 30 min buffer C, 0.7 mol/L boric acid, pH 8.7
Flow rate: 0.4 mL/min (column and post column reagent solution)
Detection: fluorescence; Ex: 331 nm, Em: 383 nm
Pressure: 16kg/cm​2
Temperature: 70 °C (column), 100 °C (post column reactor)
PC reagent: 2.5% 2-cyanoacetamide solution
Samples: disaccharides, 25 nm; monosaccharides, 50 nm:
1. cellobiose 2. maltose 3. lactose 4. rhamnose 5. lyxose 6. ribose 
7. mannose 8. fructose 9. arabinose
10. galactose 11. xylose
12. glucose

 

Analysis of Palatinit

IEX_Sugar-AXG-Fig23.png

Column: TSKgel Sugar AXG, 10 µm,
4.6 mm ID × 15 cm

Mobile phase: 0.7 mol/L borate buffer, pH 8.6
Flow rate: 0.8 mL/min
Detection: RI
Temperature: 65 °C
Injection vol.: 10 µL
Samples: 1. alpha-D-glucopyranosyl-1,6-soribitol (GPS)
2. alpha-D-glycopyranosyl-1,6-mannitol (GPM)

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
DS1072 TSKgel Sugar AX ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog