Products

HPLC Columns

TSKgel SuperQ-5PW

TSKgel SuperQ-5PW columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchange group. The resulting product exhibits very high protein capacities. TSKgel SuperQ-5PW columns are used for the separation and analysis of proteins, oligonucleotides and other biologically active molecules.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18257TSKgel SuperQ-5PW10Stainless Steel7.57.51,521
18387TSKgel SuperQ-5PW13Stainless Steel21.5155,397
          

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 10 µm and 13 µm
Pore size (mean): 100 nm
Functional group: trimethylamino
Counter ion: Cl-
pH stability: 2.0 - 12.0
Capacity (mg BSA/mL): 100
Small ion capacity: >0.13 eq/L
pKa: 12.2

 

 

 

 

         

Analysis of 16-mer Oligonucleotide

 

IEX_SuperQ-5PW-Fig11.png

 


Columns: TSKgel SuperQ-5PW,
10 µm, 7.5 mm ID × 7.5 cm

Mobile phase: A: 10 mmol/L NaOH
B: 10 mmol/L NaOH with 1 mol/L NaCl
Gradient: 0 min (0% B) 40 min (50% B)
41 min (100% B) 46 min (100% B)
Flow rate: 1 mL/min
Detection: UV @ 254 nm
Sample: 16-mer morpholine oligonucleotide, AAG AAG AAG AGG GGA G
Sample load: 0.5 O.D. (optical density)

For additional applications, please see our applications database.

 

                  

Stability of TSKgel SuperQ-5PW columns

IEX_SuperQ-5PW-Fig10A-10B.png

 

Columns: TSKgel SuperQ-5PW, 10 µm,
7.5 mm ID × 7.5 cm

Mobile phase: A: 50 mmol/L Tris-HCl, pH 8.6
B: 0.5 mmol/L sodium chloride in 50 mmol/L Tris-HCl, pH 8.6
Gradient: A-B (60 min)
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C 
Injection vol.: 100 µL
Sample load: each of 1 mg
Samples: 1. ovalbumin
2. trypsin inhibitor
Note: A. before CIP
B. after 15 times (15 days)

 

Comparison of Various Anion Exchange Columns Under Large Sample Load

IEX_SuperQ-5PW-Fig13_rev.png

 

Column: A: TSKgel SuperQ-5PW, 10 µm,
5 mm ID × 5 cm
B: Company A, Q type,
5 mm ID × 5 cm
C: Company A, perfusion Q type,
6.4 mm ID × 3 cm
All column volumes were 1.0 mL
Mobile phase: A: 50 mmol/L Tris-HCl buffer, pH 8.3
B: A + 0.5 mol/L NaCl
A to B linear gradient (60 min)
Flow rate: 0.8 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 2 mL
Samples: ovalbumin, 20 mg
trypsin inhibitor, 20 mg

Code #DescriptionLiterature Type
AN040 Selectivity studies in the analytical separation of oligonucleotides using a TSKgel SuperQ-5PW anion exchange column Application Note
DS1130 TSKgel SuperQ-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel U/HPLC Columns 2019 Product Guide -- AIEC ColumnsProduct Catalog
SR093 Column for High Performance, High-Binding Capacity Ion Exchange Chromatography: TSKgel SuperQ-5PW and Its Applications Separation Report
TP169 Selectivity studies in the analytical separation of oligonucleotides using anion exchange chromatographyTechnical Presentation
TP200 Analytical Separation Of Oligonucleotides Using Strong Anion Exchange and C18 Reversed Phase ChromatographyTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18388TSKgel SuperQ-5PW Guardgel Kit for 7.5 mm ID column20Stainless Steel 0943