TSKgel BioAssist S

Specially designed for the separation of large biomolecules such as antibodies, the large pores (130 nm) of the TSKgel BioAssist S cation exchange columns offer superior capacity and resolution at a low column pressure drop. Constructed via a polymerization technique that allows an equivalent density of sulfopropyl ionic exchange groups to be incorporated into the particle without reducing pore size, the TSKgel BioAssist S columns are unlike other ion exchange columns that use graft polymerization for polymer chain introduction. The TSKgel BioAssist S columns’ large pores are very accessible even for high molar mass proteins. This leads to higher chromatographic efficiency and binding capacity for purification.

The standard TSKgel BioAssist S column is 4.6 mm ID x 5 cm, while a 10 mm ID x 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019686	TSKgel BioAssist S	7	PEEK	4.6	5	1,704	Inquire
0021411	TSKgel BioAssist S	13	PEEK	10	10	6,491	Inquire

Product Attributes

Matrix:	polymethacrylate
Particle size (mean):	7 µm and 13 µm
Pore size (mean):	130 nm
Functional group:	sulfopropyl
Counter ion:	NA⁺
pH stability:	2.0 - 12.0
Capacity (gamma globulin):	70
Small ion capacity:	0.1 eq/L
pKa:	2.4

Analysis of Bromelain

Columns:	A: TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm B: Competitor S, 5 mm ID × 5 cm
Mobile phase:	20 min (TSKgel) or 30 min (Competitor S) linear gradient of NaCl from 0 to 0.5 mol/L in 20 mmol/L sodium phophate buffer, pH 7.0
Flow rate:	0.8 mL/min for TSKgel; 1.0 mL/min for Competitor S
Detection:	UV @ 280 nm
Temperature:	25 °C
Samples:	crude bromelein (C4882, Sigma), 1 mg in 100 L

Analysis of Protein standards

Column:	A: TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm B: Conventional S type product C, 5.0 mm ID × 5 cm C: Conventional S type product D, 4.6 mm ID × 5 cm
Mobile phase:	A: 20 mmol/L sodium phosphate buffer, pH 6.5 B: 20 mmol/L sodium phosphate buffer containing 1.0 mol/L NaCl, pH 6.5
Gradient:	32 min (A-B)
Flow rate:	0.8 mL/min
Detection:	UV @ 280 nm
Temperature:	10 °C
Injection vol.:	20 µL
Samples:	1. myoglobin 1 g/L 2. α-chymotrypsinogen, 2 g/L 3. ribonuclease A, 4 g/L 4. cytochrome C, 2 g/L 5. lysozyme, 2 g/L

Separation of IgM by Cation Exchange Chromatography

Columns:	TSKgel BioAssist S, 7 µm, 4.6 mm ID x 5 cm
Mobile phase:	20 mmol/L sodium phosphate buffer, pH 6.0
Gradient:	0 mol/L - 0.3 mol/L NaCl (5 min) 0.3 mol/L - 0.5 mol/L NaCl (10 min)
Flow rate:	1 mL/min
Detection:	UV @ 280 nm
Sample:	500 µL of 9.5 mg/mL IgM in mouse ascites fluid; shaded peaks represent albumin and IgM respectively

Analysis of Peptides

Column:	TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm
Mobile phase:	A: 20 mmol/L sodium acetate buffer, pH 5.0 B: 20 mmol/L sodium acetate buffer containing 1 mol/L NaCl, pH 5.0
Gradient:	A to B linear gradient (20 min)
Detection:	UV @ 280 nm
Temperature:	25 °C

For additional applications, please see our applications database.

Code #	Description	Literature Type
AN008	Ion Exchange Analysis of Monoclonal Antibodies on Large Pore TSKgel BioAssist Columns	Application Note
AN009	Separation of IgM using TSKgel BioAssist S	Application Note
DS1184	TSKgel BioAssist S Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
PO04	TSKgel BioAssist Ion Exchange Columns	Product Overview
SR100	TSKgel BioAssist Series Ion Exchange Columns	Separation Report
TP100	Purification of Immunoglobulin M using a novel ion-exchanger (BioAssist Q and S)	Technical Presentation
TP102	The Characteristics of Novel Semi-Preparative Ion-Exchange Columns	Technical Presentation