HPLC Columns


The TSKgel CM-5PW column is packed with porous hydrophilic polymer beads which are surface modified with carboxymethyl (CM) ligands to provide a weak cation exchange column. It is used for the separation and analysis of proteins, peptides, and other biologically active molecules.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0013068TSKgel CM-5PW10Stainless Steel7.57.51,886
0014021TSKgel CM-5PW13Stainless Steel21.5156,544

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 10 µm
Pore size (mean): 100 nm
Functional group: -CH2COO-
Counter ion: NA+
pH stability: 2.0 - 12.0
Capacity (mg Hb/mL):


Small ion capacity: >0.1 eq/L
pKa: 4.2






Selectivity of Strong and Weak TSKgel Cation Exchange Columns



Columns: TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl in 0.02 mol/L phosphate, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. trypsinogen
2. ribonuclease A
3. α-chymotrypsinogen
4. cytochrome C
5. lysozyme

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
DS1033 TSKgel CM-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02B TSKgel U/HPLC Columns 2019 Product Guide -- CIEC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0013069TSKgel CM-5PW Guardgel Kit for 7.5 mm ID column20Stainless Steel 0878