Antibody Analysis
| Columns: |
A: TSKgel CM-STAT, 7 µm, 4.6 mm ID × 10 cm
B: ProPac WCX-10, 10 µm, 4 mm ID × 25 cm
|
| Mobile phase: |
A: 20 mmol/L MES, pH 6.0
B: 20 mmol/L MES + 0.5 mol/L NaCl, pH 6.0 |
| Gradient: |
A: 0 min (10% B) 15 min (30% B) 15 min (100% B) 17 min (0% B) 17 min (10% B)
21 min (10% B)
B: 0 min (10% B) 30 min (30% B) 30 min (100% B) 32 min (100% B) 32 min (10% B) 36 min (10% B) |
| Flow rate: |
A: 1.0 mL/min B: 2.0 mL/min |
| Detection: |
UV @ 280 nm |
| Temperature: |
25 °C |
| Injection vol.: |
20 µL |
| Samples: |
monoclonal antibodies (mAb A through E) |
|
|
|
Separation of Charge Isomers
|
|
| Columns: |
TSKgel CM-STAT, 7 µm,
4.6 mm ID × 10 cm
|
| Mobile phase: |
A: 50 mmol/L sodium acetate buffer, pH 5.0
B: 30 mmol/L sodium acetate buffer (pH not adjusted)
Column equilibrated with mobile phase A, the sample is injected, then eluted stepwise to 100% mobile phase B
|
| Flow rate: |
1.0 mL/min
|
| Detection: |
UV @ 280 nm |
| Temperature: |
25 °C |
| Injection vol.:1 |
0 µL |
| Sample: |
purified mAb |
| Sample concentration: |
1 g/L |
|
For additional applications, please see our applications database.