Separation of Organic Acids
|
Columns: |
A: TSKgel OApak-A, 5 µm,
7.8 mm ID × 30 cm
B: TSKgel OApak-P guard,
6 mm ID × 4 cm |
Mobile phase: |
0.75 mmol/L sulfuric acid |
Flow rate: |
0.8 mL/min
|
Detection: |
conductivity |
Temperature: |
60 °C |
Injection vol.: |
20 µL |
Samples: |
1. pyruvic acid (50 ppm)
2. tartaric acid (500 ppm)
3. citric acid (500 ppm)
4. malic acid (500 ppm)
5. pyroglutamic acid (500 ppm)
6. lactic acid (1,000 ppm)
7. acetic acid (2,000 ppm)
8. succinic acid (1,000 ppm) |
|
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