Products

HPLC Columns

TSKgel SP-5PW

TSKgel SP-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with sulfopropyl strong cation exchange groups. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18758TSKgel SP-5PW10Stainless Steel27.51,169
07161TSKgel SP-5PW10Stainless Steel7.57.51,554
07575TSKgel SP-5PW13Stainless Steel21.5155,397
                

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 10 µm and 13 µm
Pore size (mean): 100 nm
Functional group: -(CH2)3SO3-
Counter ion: Na+
pH stability: 2.0 - 12.0
Capacity (mg Hb/mL):

40

Small ion capacity: >0.1 eq/L
pKa: 2.3

 

 

 

 

                   

Semi-preparative Purification of Lipoxidase

IEX_SP-5PW-Fig32.png

 

Column: TSKgel SP-5PW, 13 µm,
21.5 mm ID × 15 cm

Mobile phase: 120 min linear gradient from 0 mol/L to 0.5 mol/L NasSO4 in 0.02 mol/L acetate, pH 4.5
Flow rate: 4.0 mL/min
Detection: UV @ 280 nm
Recovery: lipoxidase activity collected between the two vertical lines was 84%
Sample: crude lipoxidase, 200 mg

 

Separation of Peptide Mixture

IEX_SP-5PW-Fig33.png

 

Column: TSKgel SP-5PW, 10 µm,
7.5 mm ID × 7.5 cm

Mobile phase: 30 min linear gradient from 0.02 mol/L to 0.5 mol/L phosphate, pH in 30% acetonitrile
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Injection vol.: 50 µL
Samples: 2 µg each of:
1. oxytocin
2. met-enkephalin
3. TRH
4. α-MSH
5. LH-RH (1 µg)
6. neurotensin
7. α-MSH
8. angiotensin II
9. substance P
10. β-endorphin

For additional applications, please see our applications database.

 

 

Selectivity of Strong and Weak TSKgel Cation Exchange Columns

 

IEX_CM-5PW-Fig31.png

Column: TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl in 0.02 mol/L phosphate, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. trypsinogen
2. ribonuclease A
3. α-chymotrypsinogen
4. cytochrome C
5. lysozyme


 

Code #DescriptionLiterature Type
AN025 Characterization Studies of PEGylated Lysozyme using TSKgel HPLC ColumnsApplication Note
DS1034 TSKgel SP-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02B TSKgel U/HPLC Columns 2019 Product Guide -- CIEC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
SR097 Application of TSKgel Semi-Micro for High-Sensitivity Analyses Separation Report
TP135 Characterization and Binding Capacity Studies of PEGylated LysozymeTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19308TSKgel Guard Cartridge Holder for 2 mm ID cartridges0 21416
42153TSKgel SP-5PW Guard Cartridge for 2 mm ID column, 3 pk10Stainless Steel21457
16093TSKgel SP-5PW Guardgel Kit for 21.5 mm ID column20Stainless Steel 01112
07211TSKgel SP-5PW Guardgel Kit for 7.5 mm ID column20Stainless Steel 0723