Products

HPLC Columns

TSKgel SP-NPR

The TSKgel SP-NPR column is packed with 2.5 µm, non-porous hydrophilic polymer beads of which the surface has been modified with a strong cation exchange group. Because this column contains non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality, whereas protein recovery is generally very high. 

The TSKgel SP-NPR column is used for the separation and analysis of proteins and peptides. This column is particularly useful for adeno-associated viruses and other large biopolymers.


NPR stands for Non-Porous Resin.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
13076TSKgel SP-NPR2.5Stainless Steel4.63.51,205

               

Product Attributes

Matrix: hydroxylated methacrylic polymer
Particle size (mean): 2.5 µm
Pore size (mean): non-porous
Functional group: sulfopropyl
Counter ion: NA+
pH stability: 2.0 - 12.0
Capacity (mg Hb/mL): 5
Small ion capacity: >0.1 eq/L
pKa: 2.3

 

 

 

 

                    

Analysis of Purified AAV

IEX_SP-NPR-Fig42_rev.png

 

Column: TSKgel SP-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm

Mobile phase: A. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH 7.5
B: 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH 7.5 with 0.5 mol/L NaCl; linear gradient from 20% to 100% B in 10 column volumes
Gradient: 0 min (0% B) 2 min (100% B)
Flow rate: 1 mL/min
Detection: UV @ 280 nm
Sample: purified adeno-associated virus

 

pH Gradient Analysis of Hemoglobin A1c

IEX_SP-NPR-Fig43.png

 

Column: TSKgel SP-NPR, 2.5 µm,
4.6 mm ID × 3.5 cm

Mobile phase: A: 0.02 mol/L MES, and 0.02 mol/L HEPES-NaOH, pH 6.0
B: 0.02 mol/L MES, and 0.02 mol/L HEPES-NaOH, pH 8.0
Gradient: 10 min linear gradient from 32% to 75% buffer B (pH 6.66 to pH 7.43)
Flow rate: 1.5 mL/min
Detection: VIS @ 415 nm
Sample: hemoglobin standard

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN025 Characterization Studies of PEGylated Lysozyme using TSKgel HPLC ColumnsApplication Note
DS1035 TSKgel SP-NPR ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02B TSKgel U/HPLC Columns 2019 Product Guide -- CIEC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
TP132 High Speed and High Resolution Cation Exchange Chromatography for Biological Samples on Non-Porous PackingsTechnical Presentation