Products

HPLC Columns

TSKgel SP-STAT

TSKgel SP-STAT columns are packed with 7 and 10 µm non-porous resin particles of which the surface consists of long hydrophilic chains of access network of multi-layered sulfopropyl cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.

Applications for the TSKgel SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
21964TSKgel SP-STAT7Stainless Steel4.6101,192
21963TSKgel SP-STAT10Stainless Steel33.51,127
                  

Product Attributes

Particle size (mean): 7 µm and 10 µm
Pore size (mean): nonporous
Functional group: sulfopropyl
Counter ion: Na+
pH stability: 3.0 - 10.0
Static binding capacity (mg lysozyme/g dry gel):

ca. 15 (7 µm)

ca. 10 (10 µm)

Small ion capacity:

23 µeq/g dry gel

pKa: 2.6

 

 

 

 


                     

Fast Separation of Protein Standards

IEX_SP-STAT-Fig36_rev.png

 

Column: A: TSKgel SP-STAT, 10 µm,
3.0 mm ID × 3.5 cm
B: ProSwift® SCX-1S Monolith,
4.6 mm ID × 5 cm
Mobile phase: A. 20 mmol/L sodium acetate, pH 5.0
B: 1.0 mol/L NaCl in mobile phase A, pH 5.0 for column A
1.5 mol/L NaCl in mobile phase A, pH 5.0 for column B
Gradient: 0 min (0% B) 1 min (100% B)
Flow rate: A: 2.0 mL/min
B: 4.73 mL/min
Detection: UV @ 280 nm
Samples: 1. α-chymotrypsinogen A
2. cytochrome C
3. lysozyme

 

Monitoring of Reaction Products

IEX_SP-STAT-Fig37_rev.png   

 

Column: TSKgel SP-STAT, 10 µm,
3 mm ID × 3.5 cm

Mobile phase: A: 20 mmol/L sodium acetate buffer, pH 5.0
B: 1.0 mol/L NaCl in mobile phase A, pH 5.0
Gradient: 0 min (0% B) 2 min (100% B)
Flow rate: 2.0 mL/min
Detection: UV @ 280 nm
Sample: pegylated β-lactoglobulin

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN039 Separation of mAb Isoforms Using Controlled pH Gradients and Ion Exchange Chromatography ColumnsApplication Note
AN046 UHPLC Separations using HPLC Methods and TSKgel ColumnsApplication Note
AN086 Fast Analysis Of IgG Charge Heterogeneity by Ion Exchange Chromatography Application Note
BR09 Solutions for Monoclonal Antibody Analysis and CharacterizationBrochure
DS1231 TSKgel STAT Series Cation Exchange ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02B TSKgel U/HPLC Columns 2019 Product Guide -- CIEC ColumnsProduct Catalog
PO22 TSKgel SP-STAT and TSKgel CM-STAT columnsProduct Overview
SR109 TSKgel STAT Columns for High Performance Ion Exchange Chromatography Separation Report
TP132 High Speed and High Resolution Cation Exchange Chromatography for Biological Samples on Non-Porous PackingsTechnical Presentation
TP170 Separation of mAb isoforms using controlled pH gradients and AEX/CEX columns packed with 7 and 10 μm hydrophilic non-porous resin particlesTechnical Presentation