Screening of Mouse Monoclonal Antibodies
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Column: |
TSKgel Ether-5PW, 10 μm,
8.0 mm ID × 7.5 cm, glass
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Mobile phase: |
linear gradient from A to B as shown
A: 0.05 mol/L sodium phosphate,
pH 7.0, 2.0 mol/L ammonium sulfate, 1.0 mol/L glycine
B: 0.05 mol/L sodium phosphate,
pH 7.0, 1.0 mol/L glycine
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Flow rate: |
10 mL/min |
Detection: |
UV @ 280 nm |
Samples: |
A: 20 μL unequilibrated mouse
IgG2b κ ascites
B: 20 μL unequilibrated mouse
IgM κ ascites |
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