Purification of Myristoylated Frequenin
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Column: |
TSKgel Phenyl-5PW, 10 μm,
5 mm ID × 5 cm, glass
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Mobile phase: |
equilibration in 50 mmol/L HEPES,
100 mmol/L KCI, 1 mmol/L DTT,
1 mmol/L MgCl2, 1 mmol/L CaCl2,
pH 7.5 step gradient at 12.5 min to
50 mmol/L HEPES, 1 mmol/L DTT,
1 mmol/L MgCl2, 2 mmol/L EGTA,
pH 7.5
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Flow rate: |
1 mL/min
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Detection: |
UV @ 280 nm |
Temperature: |
ambient
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Sample: |
E. coli lysate containing myristoylated frequenin, 100 μL |
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