Purification of Anti-Tumor Antibiotic
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Column: |
TSKgel Ether-5PW, 10 μm,
7.5 mm ID × 7.5 cm
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Mobile phase: |
linear gradient from 1.5 mol/L to 0 mol/L
(NH4)2SO4 in 0.1 mol/L phosphate buffer, pH 7.0
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Flow rate: |
0.8 mL/min |
Detection: |
UV @ 220 nm |
Injection vol.: |
20 μL
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Samples: |
C-1027 (red line)
C-1027-AG (blue dashed line)
concentration: 1 g/L
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