HPLC Columns

TSKgel Phenyl-5PW

TSKgel Phenyl-5PW is based on a 100 nm pore size polymethacrylate base material bonded with phenyl groups. The TSKgel Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

Being the most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules.  Besides the stainless steel standard column format, glass and PEEK column housings are available for samples that may adsorb to stainless steel.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018759TSKgel Phenyl-5PW10Stainless Steel27.51,446
0007573TSKgel Phenyl-5PW10Stainless Steel7.57.51,918
0007656TSKgel Phenyl-5PW13Stainless Steel21.5156,501

Product Attributes

Pore size (mean): 100 nm
Particle size: 10 µm and 13 µm
pH stability: 2.0 - 12.0
Functional group: phenyl




Proteins: Scale up to Preparative Separations




Column: A: TSKgel Phenyl-5PW, 10 μm,
7.5 mm ID × 7.5 cm
B: TSKgel Phenyl-5PW, 13 μm,
21.5 mm ID × 15 cm

Mobile phase: 60 min linear gradient from 1.8 mol/L to 0 mol/L (NH4)2SO4 in 0.1 mol/L phosphate buffer, pH 7.0
Flow rate: 0.5 mL/min (7.5 mm ID) or
4 mL/min (21.5 mm ID)
Detection: UV @ 280 nm
Temperature: 25 °C
Sample: 1. myoglobin
2. ribonuclease
3. lysozyme
4. α-chymotrypsinogen
5. α-chymotrypsin

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
DS1037 TSKgel Phenyl-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT03 TSKgel U/HPLC Columns 2019 Product Guide -- HIC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
SR097 Application of TSKgel Semi-Micro for High-Sensitivity Analyses Separation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0019308TSKgel Guard Cartridge Holder for 2 mm ID cartridges0 21506
0042155TSKgel Phenyl-5PW Guard Cartridge for 2 mm ID column10Stainless Steel210
0007652TSKgel Phenyl-5PW Guardgel Kit for 7.5 mm ID column20Stainless Steel 0878
0016095TSKgel Phenyl-5PW Guardgel Kit for 21.5 mm ID column20Stainless Steel 01350