TSKgel Chelate-5PW

TSKgel Chelate-5PW is based on a 100 nm pore size polymethacrylate base material covalently bonded with iminodiacetic acid (IDA) groups. Prior to chromatography, a metal ion, such as Zn²⁺, Ni²⁺ or Cu²⁺, is added to form a chelate with the IDA group. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008645	TSKgel Chelate-5PW	10	Stainless Steel	7.5	7.5	1,857	Inquire
0008646	TSKgel Chelate-5PW	13	Stainless Steel	21.5	15	7,485	Inquire

Product Attributes

Pore size (mean):	100 nm
Exclusion limit (base resin, estimate):	<1.0 × 10⁷ Da globular proteins
Ligand concentration:	20 µmol/L resin
Particle size:	10 µm and 13 µm
pH stability:	2.0 - 12.0
Functional group:	iminodiacetic acid

Structure:

10 µm

Monoclonal Antibody Purification

Column:	TSKgel Chelate-5PW (Zn²⁺), 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase:	A. 20 mmol/L Tris-HCl, pH 8.0 + 0.5 mol/L NaCl B. A + 200 mmol/L glycine
Gradient:	30 min (A-B), linear
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Sample:	Anti-HLA-A, B, C (IgG₁), NS-1 cultured supernatant

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1048	TSKgel Chelate-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT06	TSKgel U/HPLC Columns 2019 Product Guide -- AFC Columns	Product Catalog
SR074	Separation of Monoclonal Antibodies Using TSKgel HPLC Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008646	TSKgel Chelate-5PW	13	Stainless Steel	21.5	15	7485

HPLC Columns

TSKgel Chelate-5PW

Product Attributes

Structure:

Monoclonal Antibody Purification