TSKgel UP-SW2000

TSKgel UP-SW2000 columns are designed for the analysis of small proteins, peptides and oligonucleotides. The columns are packed with 2 μm silica-based beads shielded with a hydrophilic diol-type bonded phase that prevents the silica surface from interacting with protein samples.

TSKgel UP-SW2000 columns feature the same pore size as the well-established TSKgel SuperSW2000 columns. Hence methods developed using TSKgel SuperSW2000 columns can easily be transferred to TSKgel UP-SW2000 columns on conventional HPLC systems as well as on UHPLC systems.

TSKgel UP-SW2000 columns offer higher resolution, improved peak shape and increased efficiency yielding methods that are robust, reproducible and easily transferrable between UHPLC and HPLC systems.

TSKgel UP-SW2000 columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023515	TSKgel UP-SW2000	2	Stainless Steel	4.6	15	2,035	Inquire
0023514	TSKgel UP-SW2000	2	Stainless Steel	4.6	30	2,742	Inquire

Product Attributes

Base material:	silica
Particle size (mean):	2 µm
Pore size (mean):	12.5 nm
Functional group:	Diol
pH stability:	2.5 - 7.5
Calibration range (proteins):	1,000 - 1.5 ×10⁵ Da (globular proteins)

Protein Calibration Curves

TSKgel UP-SW calibration curves

Columns:	A. TSKgel UP-SW2000, 2 µm, 4.6 mm ID × 30 cm B. TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 30 cm C. TSKgel UP-SW Aggregate, 3 µm, 4.6 mm ID × 30 cm
Mobile phase:	100 mmol/L phosphate buffer, pH 6.7, + 100 mmol/L Na₂SO₄ + 0.05% NaN₃
Flow rate:	0.35 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	10 µL
Samples:	1. thyroglobulin (MW 640,000 Da) (1' thyroglobulin dimer) 2. γ-globulin (MW 155,000 Da) (2' γ-globulin dimer) 3. ovalbumin (MW 47,000 Da) 4. riboneclease A (MW 13,000 Da) 5. p-aminobenzoic acid (MW 137 Da)

Analysis of Oligonucleotides

Column:	TSKgel UP-SW2000, 2 µm, 4.6 mm ID × 30 cm × 2
Mobile phase:	0.05% NaN₃ and 0.3 mol/L NaCl in 0.05 mol/L phosphate, pH 6.7
Flow rate:	0.2 mL/min
Detection:	UV @ 260 nm
Temperature:	25 °C
Sample:	20 mer: 5’-GAATTCATCGGTTCAGAGAC-3’ 19 mer: 5’-AATTCATCGGTTCAGAGAC-3’

Analysis of Small Protein and Peptide Mixture

Column:	TSKgel UP-SW2000, 2 µm, 4.6 mm ID × 30 cm
Mobile phase:	100 mmol/L phosphate buffer, pH 6.7, 100 mmol/L Na₂SO₄, 0.05% NaN₃
Flow rate:	0.35 mL/min
Instruments:	Agilent 1100 with Chemstation v. 2.18.18
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	5 µL
Samples:	Peptide 1: Myoglobin 16.7 kDa Peptide 2: RPDFC LEPPY TGPCK ARIIR YFYNA KAGLC QTFVY GGCRA KRNNF KSAED CMRTC GGA (58 aa) 6.5 kDa Peptide 3: QRLGNQWAVGHLM (14 aa) 1.6 kDa

For additional applications, please see our applications database.

Code #	Description	Literature Type
AN115	Analysis of Oligonucleotides by SEC	Application Note
AN119	Characterization of Recombinant Protein Biotherapeutic using TSKgel UP-SW2000 Size Exclusion Chromatography Column In-Line with LenS3 MALS Detector	Application Note
AN139	Analysis of PEGylated Antibody Fragments using SEC-MALS	Application Note
DS1244	TSKgel UP-SW2000 Products	Operating Conditions and Specifications
PD21	Increased Sensitivity with LenS3 MALS Detector	Performance Data
TP227	Evaluation of a New 2 μm Silica-Based Size Exclusion Chromatography Column for the Analysis of Proteins, mAb Fragments and Peptides	Technical Presentation

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023516	TSKgel UP-SW2000 Guard Column for 4.6 mm ID column	2	Stainless Steel	4.6	2	800
0023517	TSKgel UP-SW2000 DC Guard Column for 4.6 mm ID column	2	Stainless Steel	4.6	2	800