Products

HPLC Columns

TSKgel UP-SW3000-LS

The TSKgel UP-SW3000-LS columns are dedicated to U/HPLC size exclusion analyses of monoclonal antibodies and other biopharmaceuticals combined with advanced detection such as multi-angle light scattering (MALS) or mass spectrometry (MS). The 2 µm silica particles are diol bonded for a hydrophilic surface. This, in combination with its 25 nm pore size, make the columns optimally suited for the analysis of proteins ranging between 10 kDa – 500 kDa.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
23547TSKgel UP-SW3000-LS2Stainless Steel4.6151,795
23546TSKgel UP-SW3000-LS2Stainless Steel4.6302,419

          

Product Attributes

Base material: silica
Particle size (mean): 2 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range (proteins): 10 - 500 kDa



Protein Calibration Curves


UP-SW3000-LS_calibration-curves.jpg


 


UP-SW3000-LS_protein-calibration.jpg

Columns: TSKgel UP-SW3000-LS, 2 µm, 4.6 mm ID × 30 cm
TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 30 cm
Competitor SEC, 1.7 µm, 4.6 mm ID × 30 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7,
+ 100 mmol/L Na2SO4
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Samples: 1. thyroglobulin, 640 kDa
1'. thyroglobulin dimer
2. γ-globulin,155 kDa
2'. γ-globulin dimer
3. ovalbumin 47 kDa
4. riboneclease A,13 kDa
5. p-aminobenzoic acid,137 Da

                   

Noise of buffer injection in multi-angle light scattering detection


UP-SW3000-LS_noise-comparison.jpg

Columns: A. TSKgel UP-SW3000-LS, 2 µm,
     4.6 mm ID × 30 cm

B. Competitor SEC, 1.7 µm,
    4.6 mm ID × 30 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7 +
100 mmol/L Na2SO4
Flow rate: 0.35 mL/min
Detection: Low angle light scattering (LALS) detected with LenS3 MALS detector
Temperature: 25 °C
Injection vol.: 10 µL
Sample: Mobile phase only

The key feature TSKgel UP-SW3000-LS U/HPLC columns offer is a low noise level when coupled with advanced detectors. This will save time for equilibration and improve data quality. For multi-angle light scattering (MALS) applications, a low noise level is achieved in minutes, leading to high signal-to-noise ratios and thus high sensitivity. For mass spectrometry applications, ionization efficiency by electrospray ionization is improved and increases overall MS performance and instrument uptime. This makes the TSKgel UP-SW3000-LS column ideal for in-depth analyses of aggregates and fragments of mAbs, bi- and multi-specifics.

Lot-to-lot reproducibility


 UP-SW3000-LS_lot-to-lot_reproducibility.jpg 

Column: TSKgel UP-SW3000-LS, 2 µm,
4.6 mm ID × 30 cm of three different
production lots
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7 +
100 mmol/L Na2SO4
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Injection vol.: 10 µL
Samples: Protein standard containing thyroglobulin, gamma-globulin, ovalbumin, ribonuclease A, p-amino benzoic acid, 137 Da

TSKgel UP-SW3000-LS columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.

 

Code #DescriptionLiterature Type
AN129 SEC-MALS of Antibody Therapeutics -- A Robust Method for In-Depth Sample CharacterizationApplication Note
AN132 Selecting the Optimal Column for Native SEC-MS of Monoclonal AntibodiesApplication Note
DS1246 TSKgel UP-SW3000-LS ProductsOperating Conditions and Specifications
PO50 TSKgel UP-SW3000-LS - Size Exclusion UHPLC ColumnsProduct Overview
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
23548TSKgel Guard Column UP-SW-LS2Stainless Steel4.62704
23549TSKgel Guard Column UP-SW-LS DC2Stainless Steel4.62704