Noise of buffer injection in multi-angle light scattering detection
| Columns: |
A. TSKgel UP-SW3000-LS, 2 µm,
4.6 mm ID × 30 cm
B. Competitor SEC, 1.7 µm,
4.6 mm ID × 30 cm |
| Mobile phase: |
100 mmol/L phosphate buffer, pH 6.7 +
100 mmol/L Na2SO4 |
| Flow rate: |
0.35 mL/min |
| Detection: |
Low angle light scattering (LALS) detected with LenS3 MALS detector |
| Temperature: |
25 °C |
| Injection vol.: |
10 µL |
| Sample: |
Mobile phase only |
The key feature TSKgel UP-SW3000-LS U/HPLC columns offer is a low noise level when coupled with advanced detectors. This will save time for equilibration and improve data quality. For multi-angle light scattering (MALS) applications, a low noise level is achieved in minutes, leading to high signal-to-noise ratios and thus high sensitivity. For mass spectrometry applications, ionization efficiency by electrospray ionization is improved and increases overall MS performance and instrument uptime. This makes the TSKgel UP-SW3000-LS column ideal for in-depth analyses of aggregates and fragments of mAbs, bi- and multi-specifics. |
|
Lot-to-lot reproducibility
| Column: |
TSKgel UP-SW3000-LS, 2 µm,
4.6 mm ID × 30 cm of three different
production lots |
| Mobile phase: |
100 mmol/L phosphate buffer, pH 6.7 +
100 mmol/L Na2SO4 |
| Flow rate: |
0.35 mL/min |
| Detection: |
UV @ 280 nm |
| Injection vol.: |
10 µL |
| Samples: |
Protein standard containing thyroglobulin, gamma-globulin, ovalbumin, ribonuclease A, p-amino benzoic acid, 137 Da |
TSKgel UP-SW3000-LS columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns. |