TSKgel HIC-ADC Butyl

TSKgel HIC-ADC Butyl columns utilize a novel polymer-based resin functionalized with increased ligand densities targeted specifically for Antibody-Drug Conjugates (ADCs) and monoclonal antibody separations. TSKgel HIC-ADC Butyl columns are packed with 5.0 µm non-porous particles, enhancing the separation performance for calculating Drug-to-Antibody Ratios (DAR) for ADCs.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023538	TSKgel HIC-ADC Butyl	5	Stainless Steel	4.6	3.5	1,274	Inquire
0023539	TSKgel HIC-ADC Butyl	5	Stainless Steel	4.6	10	1,488	Inquire

Product Attributes

Pore size (mean):	non-porous
Particle size (mean):	5 µm
pH stability:	2.0-12.0
Functional group:	butyl

Analysis of Antibody-Drug Conjugate (ADC) Mimic

Column:	TSKgel HIC-ADC Butyl, 5 μm, 4.6 mm ID × 10 cm
Mobile phase:	A: 0.05 mol/L sodium phosphate buffer + 1.2 mol/L ammonium sulfate (pH 7.0) B: 0.05 mol/L sodium phosphate buffer (pH 7.0) /2-propanol = 80 / 20
Gradient:	B: 0 - 100 % (0 - 15 min, linear)
Flow rate:	0.5 mL/min
Detection:	UV @ 215 nm
Temperature:	25 °C
Injection vol.:	5 μL
Sample:	SigmaMAb™ Antibody-Drug Conjugate (ADC) Mimic

Code #	Description	Literature Type
AN144	Impact of Mobile Phase Modification in HIC Chromatography for IgG and ADC Analysis	Application Note
C5500502	TSKgel HIC-ADC Butyl Products	Operating Conditions and Specifications
PO53	TSKgel HIC-ADC Butyl Columns, Designed for Antibody-Drug Conjugate Analysis	Product Overview

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023543	TSKgel HIC-ADC Guard Column	5	Stainless Steel	4.6	0.5	525

HPLC Columns

TSKgel HIC-ADC Butyl

Product Attributes

Analysis of Antibody-Drug Conjugate (ADC) Mimic