HPLC Columns

TSKgel HIC-ADC Butyl

TSKgel HIC-ADC Butyl columns utilize a novel polymer-based resin functionalized with increased ligand densities targeted specifically for Antibody-Drug Conjugates (ADCs) and monoclonal antibody separations. TSKgel HIC-ADC Butyl columns are packed with 5.0 µm non-porous particles, enhancing the separation performance for calculating Drug-to-Antibody Ratios (DAR) for ADCs.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0023538TSKgel HIC-ADC Butyl5Stainless Steel4.63.51,274
0023539TSKgel HIC-ADC Butyl5Stainless Steel4.6101,488


Product Attributes

Pore size (mean): non-porous
Particle size (mean): 5 µm
pH stability: 2.0-12.0
Functional group: butyl


Analysis of Antibody-Drug Conjugate (ADC) Mimic

Analysis of Antibody-Drug Conjugate (ADC) Mimic


Column: TSKgel HIC-ADC Butyl, 5 μm,
4.6 mm ID × 10 cm
Mobile phase: A: 0.05 mol/L sodium phosphate buffer +
     1.2 mol/L ammonium sulfate (pH 7.0)
B: 0.05 mol/L sodium phosphate buffer
     (pH 7.0) /2-propanol = 80 / 20
Gradient: B: 0 - 100 % (0 - 15 min, linear)
Flow rate: 0.5 mL/min
Detection: UV @ 215 nm
Temperature: 25 °C
Injection vol.: 5 μL
Sample: SigmaMAb™ Antibody-Drug Conjugate (ADC) Mimic

Code #DescriptionLiterature Type
AN144 Impact of Mobile Phase Modification in HIC Chromatography for IgG and ADC AnalysisApplication Note
C5500502 TSKgel HIC-ADC Butyl ProductsOperating Conditions and Specifications
PO53 TSKgel HIC-ADC Butyl Columns, Designed for Antibody-Drug Conjugate AnalysisProduct Overview
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0023543TSKgel HIC-ADC Guard Column5Stainless Steel4.60.5525