TOYOPEARL MX-Trp-650M

TOYOPEARL MX-Trp-650M is a high capacity, multimodal cation exchange resin used for the purification of monoclonal antibodies and other proteins. The tryptophan ligand has both weak carboxyl cation exchange and indole hydrophobic functional groups. The selectivity of the resin can be adjusted through control of binding or elution pH, ionic strength, salt type and additives.

TOYOPEARL MX-Trp-650M maintains dynamic binding capacity (DBC) at elevated feedstock or buffer conductivities. It maintains a high DBC even after extended CIP (clean in place) cycles using 0.5 mol/L NaOH. Applications include the separation of antibody aggregates and monomer in mAb purification polishing steps.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0022817	TOYOPEARL MX-Trp-650M	25 mL	75	247	Inquire
0022818	TOYOPEARL MX-Trp-650M	100 mL	75	772	Inquire
0022819	TOYOPEARL MX-Trp-650M	1 L	75	-	Inquire
0022820	TOYOPEARL MX-Trp-650M	5 L	75	-	Inquire

Product Attributes

Pore size (mean):	100 nm
Particle size (mean):	75 µm
Shipping buffer:	20% ethanol
pH stability:	3-13

Structure:

Purification of Monoclonal Antibodies

Separation of mAb monomers and aggregates

Resin:	TOYOPEARL MX-Trp-650M
Column size:	6.6 mm ID × 2 cm
Mobile phase:	Buffer A: 0.1 mol/L acetate + 0.2 mol/L NaCl, pH 4.3 Buffer B: 0.1 mol/L acetate + 0.4 mol/L NaCl, pH 5.6
Flow rate:	150 cm/hr (0.86 mL/min)
Detection:	UV @ 280 nm, conductivity (mS/cm)
Sample:	10 mg mAb + mAb aggregates
Sample load:	1 g/L

SEC chromatograms of the collected fractions

Resin:	TOYOPEARL MX-Trp-650M
Column size:	6.6 mm ID × 2 cm
Mobile phase:	Buffer A: 0.1 mol/L acetate + 0.2 mol/L NaCl, pH 4.3 Buffer B: 0.1 mol/L acetate + 0.4 mol/L NaCl, pH 5.6
Flow rate:	150 cm/hr (0.86 mL/min)
Detection:	UV @ 280 nm, conductivity (mS/cm)
Sample:	10 mg mAb + mAb aggregates
Sample load:	1 g/L

Stability

Stability in 0.5 mol/L NaOH

Resin:	TOYOPEARL MX-Trp-650M
Alkaline cleaning (CIP) conditions
3CV:	0.5 mol/L NaOH
5CV:	0.1 mol/L Tris-HCl, pH 8.5 + 0.3 mol/L NaCl
Flow rate:	212 cm/hr (1.0 mL/min)
DBC Measurement
Column size:	6 mm ID × 4 cm
Binding buffer:	0.05 mol/L acetate buffer, pH 4.7 + 0.1 mol/L NaCl
Flow rate:	212 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Sample:	polyclonal human IgG
Sample load:	1 mg/mL

Dynamic Binding Capacity

Effect of buffer pH and salt on DBC

Resin:	TOYOPEARL MX-Trp-650M
Column size:	6 mm ID × 4 cm
Mobile phase:	Buffer A: 0.05 mol/L acetate buffer, pH 4.0 - 6.0 + 0 - 0.2 mol/L NaCl Buffer B: 0.1 mol/L Tris-HCl buffer, pH 8.5 + 0.3 mol/L NaCl
Flow rate:	212 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Sample:	polyclonal human IgG
Sample load:	1 mg/mL

Dynamic binding capacity (DBC) calculated from 10% height of breakthrough curve

Elution Kinetics

Narrow elution peak widths

Resin:	TOYOPEARL MX-Trp-650M
Column size:	6 mm ID × 4 cm
Mobile phase:	Buffer A: 0.05 mol/L acetate buffer, pH 4.7 + 0.1 mol/L NaCl (12 mS/cm) Buffer B: 0.1 mol/L Tris-HCl buffer, pH 8.5 + 0.3 mol/L NaCl
Flow rate:	A: 212 cm/hr (1.0 mL/min) B: 424 cm/hr (2.0 mL/min) started at 124 min
Detection:	UV @ 280 nm
Sample:	CHO cell culture media, monoclonal antibody
Sample load:	1 mg/mL diluted with buffer A

Code #	Description	Literature Type
AN044	TOYOPEARL MX-Trp-650M Resin Selectivity	Application Note
AN045	TOYOPEARL MX-Trp-650M Salt Selectivity and Tolerance	Application Note
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT05	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Mixed-Mode	Product Catalog
PD11	Development of a High Capacity, Mixed-Mode Resin for High Conductivity mAb Feedstocks	Performance Data
PO32	TOYOPEARL MX-Trp-650M	Product Overview
RIS0038	TOYOPEARL MX-Trp-650M	Resin Information Sheet
TP178	A New Mixed-Mode Resin for Large Scale Biomolecule Purification	Technical Presentation

P/N	Description	Price ($)
OC41MDTRP-96	Resin Seeker TOYOPEARL MMC 96-well plate kit	-	Inquire
0045231	SkillPak Mixed-Mode 1 mL column library	341	Inquire
0045265	SkillPak Mixed-Mode 5 mL column library	469	Inquire
0045224	SkillPak TOYOPEARL MX-Trp-650M 1 mL columns (qty. 5)	284	Inquire
0045261	SkillPak TOYOPEARL MX-Trp-650M 5 mL column	235	Inquire
0022824	ToyoScreen MX-Trp-650M, 1 mL (qty. 6)	-	Inquire
0022825	ToyoScreen MX-Trp-650M, 5 mL (qty. 6)	-	Inquire
0045051	ToyoScreen RoboColumns MX-Trp-650M, 200 µL	-	Inquire
0045052	ToyoScreen RoboColumns MX-Trp-650M, 600 µL	-	Inquire