Ca⁺⁺Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules. Unlike other resins available from Tosoh Bioscience, Ca⁺⁺Pure-HA is both the ligand and the base bead.
Key applications for use of Ca⁺⁺Pure-HA include the purification of monoclonal and polyclonal antibodies, including IgG, IgA and IgM, IgG Fab regions, the purification of antibody fragments, the isolation of single-stranded from double-stranded DNA and the purification of fusion and phosphoproteins.
Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time.
Ca⁺⁺Pure-HA provides exceptional separation properties and unequalled selectivity and resolution for multiple classes of biomolecules. The highly selective and robust nature of Ca⁺⁺Pure-HA offers the flexibility to use this resin at any stage in a process from capture to final polishing.