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TSKgel SuperQ-5PW (20)

TSKgel SuperQ-5PW (20) resin is a strong anion exchange resin for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups.  This resin has a 100 nm pore size and a 20 µm bead size.

TSKgel SuperQ-5PW (20) resin is best used for high resolution process polishing steps and the capture of oligonucleotides from crude harvest. If a product with less resolution is needed, the larger particle sized TSKgel SuperQ-5PW (30) or TOYOPEARL SuperQ-650 resins may be used.  

TSKgel SuperQ-5PW resin has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.  TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins.

A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than their TOYOPEARL counterparts.

Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43383TSKgel SuperQ-5PW (20)25 mL20  336
18535TSKgel SuperQ-5PW (20)250 mL20  1,947
18546TSKgel SuperQ-5PW (20)1 L20  -
18547TSKgel SuperQ-5PW (20)5 L20  -
21920TSKgel SuperQ-5PW (20)50 L20  -
                  

Product Attributes

Base bead: 5000PW
Pore size (mean): 100 nm
Particle size (mean): 20 µm
Ligand type: strong anion
Ligand pKa: 12.2
DBC: 52 - 88 g/L
Pressure rating: 2.0 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SuperQ-5PW20-30_Structure.png

 
 
 
 

           

Purification of Oligonucleotides

1.0 mg load

IEX_SuperQ-5PW-Fig28_rev.png


Resin: TSKgel SuperQ-5PW (20)
Column size: 6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient: 50% B (2 CV)
50 - 100% B (15 CV)
100% B (2 CV)
Flow rate: 200 cm/hr (1.14 mL/min)
Detection: UV @ 254 nm
Sample: crude phosphorothioate deoxyoligonucleotide
Sample load: 1.0 mg



80% DBC elution peak with fraction purity histogram

IEX_SuperQ-5PW-Fig32_rev.png


Purification of oligonucleotide at 80% DBC

IEX_SuperQ-5PW-Fig30_rev.png


Resin: TSKgel SuperQ-5PW (20)
Column size: 6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient: 20% B (2 CV)
20 - 100% B (20 CV)
100% B (2 CV)
Flow rate: 200 cm/hr (1.14 mL/min)
Detection: UV @ 254 nm
Sample: crude phosphorothioate deoxyoligonucleotide
Sample load: 235 mg



Oligonucleotide purity and yield from 80% DBC purification


Resin: crude oligo purity final oligo purity % yield
TSKgel SuperQ-5PW (20) 66.5% 96.4% 72.5%

For additional applications, please see our applications database.

 
    

Maintains Resolution at High Oligonucleotide Load

IEX_SuperQ-5PW-Fig27.png

Resins: A & C. TSKgel SuperQ-5PW (20)
B & D. Source™ 15Q
Column size: 0.66 cm ID × 15 cm (5.1 mL)
Mobile phase: Buffer A: 20 mmol/L Tris-HCl + 10 mmol/L EDTA, pH 9.0
Buffer B: 20 mmol/L Tris-HCl + 10 mmol/L EDTA + 1.0 mol/L NaCl, pH 9.0
Flow rate: 250 cm/hr (1.43 mL/min)
Detection: UV @ 254 nm
Sample: DNA based oligonucleotides
Sample load: A & B. 1 mg/column
C & D. 20 mg/column
Separation conditions: Column was washed with 5 CV 100% buffer A followed by 11 mL injection. Column was then washed with 3 CV 100% buffer A followed by 6 CV of linear gradient 35-53 buffer B. Finally, column was washed with 5 CV 100% buffer B.
Fractions: 0.5 mL fractions were taken from peaks of interest and analyzed on a TSKgel DNA-NPR column.

    

Scale Up or Down using the Same Ligand


IEX_SuperQ-5PW-Fig1.png

Resins: A. TOYOPEARL SuperQ-650C, 100 µm
B. TOYOPEARL SuperQ-650M, 65 µm
C. TOYOPEARL SuperQ-650S, 35 µm
D. TSKgel SuperQ-5PW (30), 30 µm
E. TSKgel SuperQ-5PW (20), 20 µm
F. TSKgel SuperQ-5PW, 10 µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, × 5)
Load volume: 100 µL 


Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD09 Purification of DNA-Based Oligonucleotide at 60°C on TSKgel SuperQ-5PW (20) ResinPerformance Data
PD10 Increased Load of DNA-Based Oligonucleotide on TSKgel SuperQ-5PW (20) ResinPerformance Data
RIS0032 TSKgel SuperQ-5PWResin Information Sheet
TP121 High Resolution Anion Exchange Chromatography Purification of OligonucleotidesTechnical Presentation
TP136 Purification of oligonucleotides using anion-exchange chromatographyTechnical Presentation
TP138 One-step oligonucleotide purifications using anion-exchange chromatography resins Technical Presentation
TP168 Purification of Oligonucleotides Using High Resolution Strong Anion Exchange Chromatography ResinsTechnical Presentation
TP169 Selectivity studies in the analytical separation of oligonucleotides using anion exchange chromatographyTechnical Presentation
TP174 Chromatographic Purification of Oligonucleotide-Based Biotherapeutic ProductsTechnical Presentation
P/NDescriptionPrice ($) 
45208SkillPak TSKgel SuperQ-5PW (20) 1 mL columns (qty. 5)277
45244SkillPak TSKgel SuperQ-5PW (20) 5 mL column234
43380TSKgel IEXPAK PW, 20 µm 940