Process Media


TOYOPEARL GigaCap S-650M resin is a high capacity cation exchange resin optimized for the capture and purification of both small and large proteins, including monoclonal antibodies.  TOYOPEARL GigaCap S-650M resin is composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with sulfopropyl (S) strong cation exchange groups. TOYOPEARL GigaCap-650M has a 100 nm pore size and a 75 µm particle size and can be used for high throughput capture, intermediate purification, and polishing process steps.

TOYOPEARL GigaCap S-650M exhibits typical dynamic binding capacities greater than 150 g/L for human IgG, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

TOYOPEARL GigaCap S-650M resin has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0021833TOYOPEARL GigaCap S-650M100 mL75  525
0021834TOYOPEARL GigaCap S-650M250 mL75  977
0021835TOYOPEARL GigaCap S-650M1 L75  -
0021836TOYOPEARL GigaCap S-650M5 L75  -
0021837TOYOPEARL GigaCap S-650M50 L75  -

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 75 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: 80 - 120 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life (estimated): 10 years





Dynamic Binding Capacity at Various Linear Velocities


Resin: TOYOPEARL GigaCap S-650M
Column size: 6.0 mm ID × 4 cm (1.13 mL)
Mobile phase: 0.1 mol/L acetate buffer, pH 4.7
Flow rates: 212, 424, 636, 1060 cm/hr (1.0, 2.0, 3.0, 5.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG (1.0 g/L)

Pressure-Flow Curves


TOYOPEARL GigaCap S-650M was packed into a 36 cm ID × 25 cm bed height Eastern Rivers BioStream column to measure the pressure-flow characteristics. The resin had similar profiles when packed and run in both H2O and 1.0 mol/L NaCl.

Elution Pool Volume Comparison


Resins: TOYOPEARL GigaCap S-650M
Capto S
Column size: 6.0 mm ID × 4 cm
Mobile phase: Buffer A: 0.1 mol/L acetate buffer,
pH 4.7
Buffer B: 0.1 mol/L acetate buffer,
pH 4.7 +1.0 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG (1.0 mg/mL)

Code #DescriptionLiterature Type
AN012 TOYOPEARL GigaCap S-650M-A very high capacity cation exchange resinApplication Note
AN069 TOYOPEARL GigaCap Cation Exchange Resins for Aggregate Removal from Monoclonal Antibodies Application Note
BR04 TOYOPEARL GigaCap Ion Exchange ResinsBrochure
BR06 TOYOPEARL GigaCap Resins for Reducing Downstream WFI ConsumptionBrochure
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD16 Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl AlcoholPerformance Data
PO08 TOYOPEARL GigaCap S-650M for High Capacity Cation Exchange Chromatography of IgGProduct Overview
RIS0024 TOYOPEARL GigaCap-S-650Resin Information Sheet
TP119 A High Capacity Strong Cation Exchange Resin for the Chromatographic Purification of Monoclonal Antibodies and Other ProteinsTechnical Presentation
TP123 Novel High Binding Capacity Ion Exchange Resins for Biochromatography Technical Presentation
TP145 Purification of Monoclonal Antibodies Using Cation Exchange TOYOPEARL GigaCap ResinsTechnical Presentation
P/NDescriptionPrice ($) 
0045101MiniChrom TOYOPEARL GigaCap S-650M245
OC41MDCEX-96Resin Seeker TOYOPEARL CIEX 96-well plate kit426
OC41MDGCS-650MResin Seeker TOYOPEARL GigaCap S-650M 96-well plate kit317
0021868ToyoScreen GigaCap S-650M, 1 mL (qty. 6)-
0021869ToyoScreen GigaCap S-650M, 5 mL (qty. 6)-
0021394ToyoScreen IEC Cation Mix Pack, 1 mL (qty. 5)-
0021395ToyoScreen IEC Cation Mix Pack, 5 mL (qty. 5)-
0021396ToyoScreen IEC Mix Pack, 1 mL (qty. 5)-
0021397ToyoScreen IEC Mix Pack, 5 mL (qty. 5)-
0045001ToyoScreen RoboColumns GigaCap S-650M, 200 µL251
0045002ToyoScreen RoboColumns GigaCap S-650M, 600 µL294