Process Media


TOYOPEARL HW-40C resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-40C resin has a 5 nm pore size and a 100 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-40C resin useful for protein separations. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.


The good mechanical stability of the TOYOPEARL HW-40C resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-40C resin is chemically stable from pH

2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.


If higher resolution is needed, the smaller particle TOYOPEARL HW-40F and TOYOPEARL HW-40S resins are preferred for process SEC. For desalting, where the resin is used in a filtration mode to remove the target from a buffer, TOYOPEARL HW-40EC can also employed in place of TOYOPEARL HW-40C due to its better flow dynamics at lower operating pressures.


P/NDescriptionResin VolumeParticle Size (µm)   Price
0019807TOYOPEARL HW-40C150 mL75  233
0007449TOYOPEARL HW-40C500 mL75  523
0014683TOYOPEARL HW-40C1 L75  -
0007969TOYOPEARL HW-40C5 L75  -
0021484TOYOPEARL HW-40C50 L75  -


Product Attributes

Pore size (mean):  5 nm 
Exclusion limit: 10,000 Da
Particle size (mean): 75 µm
Shipping buffer: 20% ethanol
pH stability: 2-13
Stability data: 10 years


Molar Mass Separation Ranges

Polyethylene glycols
and oxides
100 - 3,000 Da
Dextrans 100 - 7,000 Da
Globular proteins 100 - 1 × 10Da




Calibration Curves for Globular Proteins


Resins: A. TOYOPEARL HW-40
Column size: 22 mm ID × 30 cm
Mobile phase: 0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection: UV @ 280 nm
Temperature: ambient
Samples: protein standards
Legend: Ve = elution volume
Vc = column volume
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT01 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion Product Catalog
RIS0040 TOYOPEARL HW-40Resin Information Sheet