TOYOPEARL HW-55F

TOYOPEARL HW-55F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-55F resin has a 50 nm pore size and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-55F resin useful for protein separations of biomolecules between 1,000 and 700,000 Da in size.

The good mechanical stability of the TOYOPEARL HW-55F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-55F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

If higher resolution is needed, the smaller particle TOYOPEARL HW-55S resin can also be used for process SEC.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0019812	TOYOPEARL HW-55F	150 mL	45	277	Inquire
0007457	TOYOPEARL HW-55F	500 mL	45	640	Inquire
0014687	TOYOPEARL HW-55F	1 L	45	-	Inquire
0008063	TOYOPEARL HW-55F	5 L	45	-	Inquire
0021918	TOYOPEARL HW-55F	50 L	45	-	Inquire

Product Attributes

Pore size (mean):	50 nm
Exclusion limit:	700,000 Da
Particle size (mean):	45 µm
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data:	10 years

Molar Mass Separation Ranges

Polyethylene glycols and oxides	100 - 1.5 × 10⁵Da
Dextrans	1,000 - 2 × 10⁵Da
Globular proteins	1,000 - 7 × 10⁵Da

Structure:

Calibration Curves for Globular Proteins

Resins:	A. TOYOPEARL HW-40 B. TOYOPEARL HW-50 C. TOYOPEARL HW-55 D. TOYOPEARL HW-65 E. TOYOPEARL HW-75
Column size:	22 mm ID × 30 cm
Mobile phase:	0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection:	UV @ 280 nm
Temperature:	ambient
Samples:	protein standards
Legend:	V_e= elution volume V_c= column volume

Antibody Separation

Elution profiles of colostral whey

Resin:	TOYOPEARL HW-55F
Column size:	17 mm ID × 64 cm
Mobile phase:	25 mmol/L Tris-HCl with 0.3 mol/L NaCl, pH 7.5
Flow rate:	18 cm/hr (0.7 mL/min)
Detection:	A. UV @ 280 nm B. UV @ 254 nm
Temperature:	23 °C
Samples:	1. unknown 2. IgG₁ 3. serum albumin 4. β-lactoglobulin (dimer) 5. α-lactalbumin 6-12. unknown

Recovery of Activity

Purification of crude β-galactosidase

Resin:	TOYOPEARL HW-55F
Column size:	25 mm ID × 55 cm × 2
Mobile phase:	0.2 mol/L phosphate, pH 6.7
Flow rate:	12 cm/hr (1.0 mL/min)
Detection:	A. UV @ 280 nm B. enzymatic activity
Temperature:	22 °C
Sample:	3 mL containing 45 mg of crude β-galactosidase

Fraction	Yield (%)	Specific activity (units/mg)	Degree of purification
Original sample		0.95
1	94	2.8	2.9 ×
2	93	3.7	3.9 ×
3	83	6.4	6.7 ×

For additional applications, please see our applications database.

Comparison of the Elution Volumes of Proteins in Presence and Absence of NaCl

Resin:	TOYOPEARL HW-55F
Column size:	22 mm ID × 50 cm
Mobile phase:	25 mmol/L Tris-HCl with (•) or without (◌) 0.5 mol/L NaCl, pH 7.5
Flow rate:	16 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm, 420 nm for heme proteins, 200 nm for proteins without aromatic amino acids
Temperature:	5-10 °C

Code #	Description	Literature Type
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT01	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion	Product Catalog
RIS0042	TOYOPEARL HW-55	Resin Information Sheet

P/N	Description	Price ($)
0019819	TOYOPEARL SECPAK HMW	538	Inquire
0019821	TOYOPEARL SECPAK LMW	538	Inquire