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TOYOPEARL HW-75F

TOYOPEARL HW-75F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-75F resin has a pore size of > 100 nm and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-75F resin useful for protein separations of biomolecules between 500,000 and 50,000,000 Da in size.

 

The good mechanical stability of the TOYOPEARL HW-75F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-75F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If higher resolution is needed, the smaller particle TOYOPEARL HW-75S resin can also be used for process SEC.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19816TOYOPEARL HW-75F150 mL45  274
07469TOYOPEARL HW-75F500 mL45  624
14691TOYOPEARL HW-75F1 L45  -
08072TOYOPEARL HW-75F5 L45  -
              

Product Attributes

Pore size (mean): >100 nm
Exclusion limit: 50,000,000 Da
Particle size (mean): 45 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

 

Molar Mass Separation Ranges

Polyethylene glycols
and oxides
4,000 - 5 × 10Da
Dextrans 1 × 105 - 1 × 10Da
Globular proteins 5 × 105 - 5 × 10Da

Structure:

SEC_HWresin_structure.png

 
 

Calibration Curves for Globular Proteins

SEC_HW-40_HW-50_HW55-Fig2_rev.png

Resins: A. TOYOPEARL HW-40
B. TOYOPEARL HW-50
C. TOYOPEARL HW-55
D. TOYOPEARL HW-65
E. TOYOPEARL HW-75
Column size: 22 mm ID × 30 cm
Mobile phase: 0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection: UV @ 280 nm
Temperature: ambient
Samples: protein standards
Legend: Ve= elution volume
Vc= column volume
 
 
    

Separation of pBR322 DNA

SEC_HW-75F-Fig11_rev.png

Resin: TOYOPEARL HW-75F
Column size: 16 mm ID × 130 cm
Mobile phase: 10 mmol/L Tris-HCl, 1 mmol/L EDTA, 0.2 mol/L NaCL, pH 8
Flow rate: 7 cm/hr (0.23 mL/min)
Detection: UV @ 260 nm
Temperature: ambient
Sample: A. crude plasmid DNA extract
B. DNA from A
C. DNA from hydroxyapatite chromatography

For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT01 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion Product Catalog
RIS0044 TOYOPEARL HW-75Resin Information Sheet
P/NDescriptionPrice ($) 
19819TOYOPEARL SECPAK HMW446