Product Attributes
| Base bead: |
HW-75 |
| Pore size (mean): |
> 100 nm |
| Particle size (mean): |
45 µm |
| Ligand type: |
weak anion |
| Ligand pKa: |
8.5 |
| DBC: |
> 70 g/L |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data (estimated): |
7 years* |
|
|
Structure:
|
* Testing is on-going to obtain data for longer stability data timepoints.
Aggregate Removal
Separation of aggregates from IgG1 monomer
| Resin: |
TOYOPEARL NH2-750F
|
| Column size: |
5 cm ID × 5 cm |
| Mobile phase: |
A: 20 mmol/L Tris-HCl, pH 8.0
B: mobile phase A + 1.0 mol/L NaCl
|
| Gradient: |
0 - 100% B (60 min) |
| Flow rate: |
300 cm/hr (1.0 mL/min) |
| Detection: |
UV @ 280 nm |
| Temperature: |
ambient |
| Sample: |
mAb (IgG1), 0.5 g/L |
|
|
Flow-through removal of aggregates from mAb monomer
| Resin: |
TOYOPEARL NH2-750F
|
| Column size: |
6.6 mm ID × 6 cm |
| Mobile phase: |
A: 10 mmol/L Tris-HCl, 250 mmol/L NaCl, pH 7.0
B: 1.0 mol/L NaOH (CIP)
|
| Gradient: |
isocratic |
| Flow rate: |
300 cm/hr (1.71 mL/min) |
| Detection: |
UV @ 280 nm, conductivity |
| Temperature: |
ambient |
| Sample: |
mAb (IgG1), 1.0 g/L |
|
For additional applications, please see our applications database.