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TOYOPEARL NH2-750F

TOYOPEARL NH2-750F media is a salt tolerant anion exchange resin capable of aggregate removal in both flow-through and binding and elution modes. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with proprietary primary amine (NH2) strong anion exchange groups.

TOYOPEARL NH2-750F resin is ideal for process scale applications from the capture of proteins from biological feedstock (mammalian cell culture, plasma, bacterial feedstock, etc.) without dilution to the intermediate or final purification of monoclonal antibodies (mAbs) where aggregates and other impurities are removed from the target of interest. 

TOYOPEARL NH2-750F resin is available in one particle size, 45 µm, which corresponds to the F-grade designation.

TOYOPEARL NH2-750F exhibits typical binding capacities in the vicinity of 70 g/L for BSA, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.   

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
23438TOYOPEARL NH2-750F100 mL45  497
23439TOYOPEARL NH2-750F250 mL45  925
23440TOYOPEARL NH2-750F1 L45  -
23441TOYOPEARL NH2-750F5 L45  -
23442TOYOPEARL NH2-750F50 L45  -
                 

Product Attributes

Base bead: HW-75
Pore size (mean): > 100 nm
Particle size (mean): 45 µm
Ligand type: weak anion
Ligand pKa: 8.5
DBC: > 70 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (estimated): 10 years

Structure:

IEX_NH2-750F_Structure.png

 
 
 
 

           

Aggregate Removal

Separation of aggregates from IgG1 monomer

IEX_NH2-750F-Fig9A.png

Resin: TOYOPEARL NH2-750F
Column size: 5 cm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.0
B: mobile phase A + 1.0 mol/L NaCl
Gradient: 0 - 100% B (60 min)
Flow rate: 300 cm/hr (1.0 mL/min) 
Detection: UV @ 280 nm
Temperature: ambient
Sample: mAb (IgG1), 0.5 g/L

Flow-through removal of aggregates from mAb monomer

IEX_NH2-750F-Fig9B.png

Resin: TOYOPEARL NH2-750F
Column size: 6.6 mm ID × 6 cm
Mobile phase: A: 10 mmol/L Tris-HCl, 250 mmol/L NaCl, pH 7.0
B: 1.0 mol/L NaOH (CIP)
Gradient: isocratic
Flow rate: 300 cm/hr (1.71 mL/min) 
Detection: UV @ 280 nm, conductivity
Temperature: ambient
Sample: mAb (IgG1), 1.0 g/L

For additional applications, please see our applications database.

 
           

Alkaline Stability

IEX_NH2-750F_Fig10.png


Pressure-Flow Curves

IEX_NH2-750F_Fig7.png

Resin: TOYOPEARL NH2-750F
Column size: 4.4 cm ID × 29 cm
Mobile phase: 0.1 mol/L NaCl
Flow rate: multiple

Salt Tolerance Comparison

IEX_NH2-750F-Fig8.png

Resins: TOYOPEARL NH2-750F
TOYOPEARL Q-600C AR
TOYOPEARL GigaCap Q-650M
TOYOPEARL SuperQ-650M
TOYOPEARL DEAE-650M
Column size: 5 cm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.0
B: mobile phase A + 2.0 mol/L NaCl
Gradient: 0 - 100% B (120 min)
Flow rate: 300 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: BSA (1.0 g/L)

Code #DescriptionLiterature Type
AN066 TOYOPEARL NH2-750F Aggregate Removal from Monoclonal AntibodiesApplication Note
AN091 TOYOPEARL NH2-750F Flow-through Aggregate Removal from Monoclonal Antibodies Application Note
AN094 Effective Removal of Endotoxins with AEX Resin TOYOPEARL NH2-750FApplication Note
AN116 Reduce Downstream Processing Costs for mAbs by Switching to a Tosoh 2-Step PlatformApplication Note
BR10 Best-In-Class Bioprocessing ResinsBrochure
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PO39 TOYOPEARL NH2-750F, a Salt Tolerant Anion Exchange ResinProduct Overview
RIS0036 TOYOPEARL NH2-750FResin Information Sheet
TP217 Evaluation of Viral Clearance for Monoclonal Antibody Chromatographic Process StepsTechnical Presentation
P/NDescriptionPrice ($) 
OC41MDNH2-750FResin Seeker TOYOPEARL NH2-750F 96-well plate kit286
45226SkillPak AEX 1 mL column library305
45228SkillPak Antibody 1 mL column library442
45232SkillPak Best in Class 1 mL column library 391
45266SkillPak Best in Class 5 mL column library1,330
45229SkillPak mAb Platform 1 mL column library437
45263SkillPak mAb Platform 5 mL column library757
45230SkillPak Salt Tolerant 1 mL column library305
45264SkillPak Salt Tolerant 5 mL column library419
45209SkillPak TOYOPEARL NH2-750F 1 mL columns (qty. 5)253
45245SkillPak TOYOPEARL NH2-750F 5 mL column210
23443ToyoScreen NH2-750F, 1 mL (qty. 6)173
23444ToyoScreen NH2-750F, 5 mL (qty. 6)360
45021ToyoScreen RoboColumns NH2-750F, 200 µL239
45022ToyoScreen RoboColumns NH2-750F, 600 µL280