Process Media


TOYOPEARL GigaCap Q-650M is a high capacity, high resolution anion exchange resin optimized for the capture and purification of both small and large proteins, including oligonucleotides and monoclonal antibodies.  The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with quaternary amine (Q) strong anion exchange groups. This resin has a 100 nm pore size and a 75 µm bead size.

TOYOPEARL GigaCap Q-650M exhibits typical dynamic binding capacities in the vicinity of 175 mg/mL for BSA and 49 mg/mL for thyroglobulin, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

TOYOPEARL GigaCap Q-650M media has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.


P/NDescriptionResin VolumeParticle Size (µm)   Price
21854TOYOPEARL GigaCap Q-650M100 mL75  471
21855TOYOPEARL GigaCap Q-650M250 mL75  877
21856TOYOPEARL GigaCap Q-650M1 L75  -
21857TOYOPEARL GigaCap Q-650M5 L75  -
21858TOYOPEARL GigaCap Q-650M50 L75  -

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 75 µm
Ligand type: strong anion
Ligand pKa: 12.2
DBC: 105 - 155 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life (estimated): 10 years





Dynamic Binding Capacity at 212 cm/hr


Pressure-Flow Curves


Resins: TOYOPEARL GigaCap Q-650M
Column size: 22 mm ID × 20 cm
Mobile phase: H2O

Elution Pool Volume Comparison


Resins: TOYOPEARL GigaCap Q-650M
Capto Q
Column size: 6.0 mm ID × 4 cm
Mobile phase: Buffer A: 15 mmol/L Tris-HCl,
pH 8.7
Buffer B: 15 mmol/L Tris-HCl,
pH 8.7 + 1.0 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG (1.0 g/L)

Code #DescriptionLiterature Type
BR04 TOYOPEARL GigaCap Ion Exchange ResinsBrochure
BR06 TOYOPEARL GigaCap Resins for Reducing Downstream WFI ConsumptionBrochure
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD16 Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl AlcoholPerformance Data
PO16 TOYOPEARL GigaCapQ-650M for high capacity Anion Exchange Chromatography of small and large proteinsProduct Overview
RIS0023 TOYOPEARL GigaCap-Q-650Resin Information Sheet
TP123 Novel High Binding Capacity Ion Exchange Resins for Biochromatography Technical Presentation
TP136 Purification of oligonucleotides using anion-exchange chromatographyTechnical Presentation
TP138 One-step oligonucleotide purifications using anion-exchange chromatography resins Technical Presentation
P/NDescriptionPrice ($) 
OC41MDAEX-96Resin Seeker TOYOPEARL AIEX 96-well plate kit384
OC41MDGCQ-650MResin Seeker TOYOPEARL GigaCap Q-650M 96-well plate kit286
45226SkillPak AEX 1 mL column library305
45228SkillPak Antibody 1 mL column library442
45203SkillPak TOYOPEARL GigaCap Q-650M 1 mL columns (qty. 5)253
45239SkillPak TOYOPEARL GigaCap Q-650M 5 mL column210
21859ToyoScreen GigaCap Q-650M, 1 mL (qty. 6)165
21860ToyoScreen GigaCap Q-650M, 5 mL (qty. 6)341
21392ToyoScreen IEC Anion Mix Pack, 1 mL (qty. 5)160
21393ToyoScreen IEC Anion Mix Pack, 5 mL (qty. 5)334
21396ToyoScreen IEC Mix Pack, 1 mL (qty. 5)160
21397ToyoScreen IEC Mix Pack, 5 mL (qty. 5)334
45003ToyoScreen RoboColumns GigaCap Q-650M, 200 µL239
45004ToyoScreen RoboColumns GigaCap Q-650M, 600 µL280