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TOYOPEARL GigaCap Q-650S

TOYOPEARL GigaCap Q-650S is a high capacity, high resolution anion exchange resin optimized for the capture and purification of both small and large proteins, including oligonucleotides and monoclonal antibodies.  The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with quaternary amine (Q) strong anion exchange groups. This resin has a 100 nm pore size and a 35 µm bead size.

TOYOPEARL GigaCap Q-650S has dynamic binding capacities approaching 190 g/L for bovine serum albumin, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  The high capacity, combined with the 35 µm mean particle size, makes TOYOPEARL GigaCap Q-650S ideal for high resolution applications.

TOYOPEARL GigaCap Q-650 media has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
22881TOYOPEARL GigaCap Q-650S25 mL35  229
22882TOYOPEARL GigaCap Q-650S250 mL35  1,702
22883TOYOPEARL GigaCap Q-650S1 L35  -
22884TOYOPEARL GigaCap Q-650S5 L35  -
22885TOYOPEARL GigaCap Q-650S50 L35  -
                 

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 35 µm
Ligand type: strong anion
Ligand pKa: 12.2
DBC: > 170 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life (estimated): 10 years

Structure:

IEX_GigaCap-Q-650M-S_Structure.png

 
 
 
 

           

Purification of Oligonucleotides

1.0 mg load

IEX_GigaCap-Q-650-Fig29_rev.png


Resin: TOYOPEARL GigaCap Q-650S
Column size: 6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient: 50% B (2 CV)
50 - 100% B (15 CV)
100% B (2 CV)
Flow rate: 200 cm/hr (1.14 mL/min)
Detection: UV @ 254 nm
Sample: crude phosphorothioate deoxyoligonucleotide
Sample load: 1.0 mg



80% DBC elution peak with fraction purity histogram

IEX_GigaCap-Q-650-Fig33_rev.png


Purification of oligonucleotide at 80% DBC

IEX_GigaCap-Q-650-Fig31_rev2.png


Resin: TOYOPEARL GigaCap Q-650S
Column size: 6.6 mm ID × 18 cm (6.16 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient: step to 20% B (2 CV)
20 - 100% B (20 CV)
100% B (2 CV)
Flow rate: 200 cm/hr (1.14 mL/min)
Detection: UV @ 254 nm
Sample: crude phosphorothioate deoxyoligonucleotide
Sample load: 181.4 mg



Oligonucleotide purity and yield from 80% DBC purification


Resin: crude oligo purity final oligo purity % yield
TOYOPEARL GigaCap
Q-650S
66.5% 96.9% 81.3%

For additional applications, please see our applications database.

 
           

High DBC at Increased Linear Velocities

IEX_GigaCapQ650S_DBC.png

Resin: TOYOPEARL GigaCap Q-650S
Column size: 6.0 mm ID × 4 cm
Mobile phase: A: 50 mmol/L Tris-HCl buffer,
pH 8.5
B: mobile phase A + 0.5 mol/L NaCl
Linear velocity: 201, 424, and 585 cm/hr
Residence time: 1.2 min, 0.6 min, and 0.4 min
Detection: UV @ 280 nm
Sample: BSA (1.0 g/L)

Pressure-Flow Curves Comparison

IEX_GigaCap-Q-650-Fig24_rev.png

Resin: TOYOPEARL GigaCap Q-650S
Column size: 22 mm ID × 20 cm
Mobile phase: 0.1 mol/L NaCl
Detection: pressure (MPa)

Code #DescriptionLiterature Type
AN050 Purification of Oligonucleotides on TOYOPEARL GigaCap Q-650SApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PO36 TOYOPEARL GigaCap Q-650SProduct Overview
RIS0023 TOYOPEARL GigaCap-Q-650Resin Information Sheet
TP168 Purification of Oligonucleotides Using High Resolution Strong Anion Exchange Chromatography ResinsTechnical Presentation
TP174 Chromatographic Purification of Oligonucleotide-Based Biotherapeutic ProductsTechnical Presentation
TP192 Characterization of Two Novel High Capacity, High Resolution Strong Ion Exchange Resins Technical Presentation
P/NDescriptionPrice ($) 
45105MiniChrom TOYOPEARL GigaCap Q-650S245
45025ToyoScreen RoboColumns GigaCap Q-650S, 200 µL239
45026ToyoScreen RoboColumns GigaCap Q-650S, 600 µL280