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Process Media

TOYOPEARL SuperQ-650S

TOYOPEARL SuperQ-650S resin is a strong anion exchanger for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups.  This resin has a 100 nm pore size and a 35 µm bead size.

 TOYOPEARL SuperQ-650S resin is best used for high resolution intermediate purification and polishing process steps or for the capture of oligonucleotides. If greater resolution is needed, the smaller particle size TOYOPEARL SuperQ-650S or TSKgel SuperQ-5PW resins may be used. If less resolution is desired, the TOYOPEARL SuperQ-650M or TOYOPEARL SuperQ-650C resins may be used.

As both the TOYOPEARL SuperQ-650 and TSKgel SuperQ-5PW resins use the same ligand and backbone chemistry, the selectivity will be unchanged between the two resin types.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19823TOYOPEARL SuperQ-650S25 mL35  184
17223TOYOPEARL SuperQ-650S250 mL35  1,333
17224TOYOPEARL SuperQ-650S1 L35  -
17225TOYOPEARL SuperQ-650S5 L35  -
19679TOYOPEARL SuperQ-650S50 L35  -
                  

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 35 µm
Ligand type: strong anion
Ligand pKa: 12.2
DBC: 105 - 155 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SuperQ-650C-M-S_Structure.png

 
 
 
 

    

Scale Up or Down using the Same Ligand


IEX_SuperQ-5PW-Fig1.png

Resins: A. TOYOPEARL SuperQ-650C, 100 µm
B. TOYOPEARL SuperQ-650M, 65 µm
C. TOYOPEARL SuperQ-650S, 35 µm
D. TSKgel SuperQ-5PW (30), 30 µm
E. TSKgel SuperQ-5PW (20), 20 µm
F. TSKgel SuperQ-5PW, 10 µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, × 5)
Load volume: 100 µL 


Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0029 TOYOPEARL SuperQ-650Resin Information Sheet
TP168 Purification of Oligonucleotides Using High Resolution Strong Anion Exchange Chromatography ResinsTechnical Presentation
TP174 Chromatographic Purification of Oligonucleotide-Based Biotherapeutic ProductsTechnical Presentation
P/NDescriptionPrice ($) 
45206SkillPak TOYOPEARL SuperQ-650S 1 mL columns (qty. 5)253
45242SkillPak TOYOPEARL SuperQ-650S 5 mL column210
19817TOYOPEARL IEXPAK HP427